Structural changes induced by tetracycline in secretory ameloblasts in young rats.

The effects of tetracycline hydrochloride (TC) on secretory ameloblasts in mandibular incisors and first molars in young rats were investigated. The pathogenesis of the TC-induced changes was examined in light and electron microscopes 6, 12, 24 and 96 h after a single intraperitoneal injection of a high dose of TC (200 mg TC/kg body wt.) into 4-d-old animals. Secretory ameloblasts from non-injected animals served as controls. Cellular changes were observed in all teeth 6 h after injection. The changes increased with the postinjection period and after 96 h they were so severe that they were considered irreversible. The incipient changes of the ameloblasts comprised altered structure and location of the mitochondria, succeeded by dilated cisternae of endoplasmic reticulum arranged in short curled formations. Later on, distorted Golgi complexes were observed. Secretory granules appeared in various numbers in the Golgi zone and in the Tomes' processes. In severely affected ameloblasts the cell nuclei showed an increased content of chromatin. Autophagic vacuoles and extracellular membrane bound fragments with cellular remnants were frequent, indicating death of some of the ameloblasts. Areas of stippled secretory material were observed along the formative enamel front and in extracellular spaces between the ameloblasts. In the 96-h group calcified bodies with a varying content of crystals were scattered between the cells. Incisors and the molars displayed similar patterns of structural changes of the secretory ameloblasts; however, the changes developed earlier and became more severe in the molars.