Literature DB >> 6935666

Purification and properties of the double-stranded RNA-activated eukaryotic initiation factor 3 kinase from rabbit reticulocytes.

H Grosfeld, S Ochoa.   

Abstract

The double-stranded RNA (dsRNA)-activated protein kinase (DAI) that phosphorylates the alpha subunit of the eukaryotic initiation factor eIF-2 and inhibits chain initiation has been isolated from rabbit reticulocyte lysates. The nonactivated enzyme or the enzyme partially activated by incubation with low levels of dsRNA (pro-DAI) could be purified only to a slight extent. However, the enzyme that was fully activated by incubation with both dsRNA and ATP was purified to near homogeneity. Active DAI is a phosphoprotein with an apparent subunit mass of 68,000 daltons. It can phosphorylate histone as well as the alpha subunit of eIF-2. Our results suggest that, after interaction with dsRNA, the enzyme phosphorylates itself and is thereby activated to phosphorylate alpha eIF-2 and histone.

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Year:  1980        PMID： 6935666      PMCID： PMC350318          DOI： 10.1073/pnas.77.11.6526

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  pppA2'p5'A2'p5'A: an inhibitor of protein synthesis synthesized with an enzyme fraction from interferon-treated cells.

Authors:  I M Kerr; R E Brown
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

3.  Phosphorylation of eukaryotic protein synthesis initiation factors.

Authors:  R Benne; J Edman; R R Traut; J W Hershey
Journal:  Proc Natl Acad Sci U S A       Date:  1978-01       Impact factor: 11.205

4.  Control of globin synthesis by hemin: factors influencing formation of an inhibitor of globin chain initiation in reticulocyte lysates.

Authors:  M Gross; M Rabinovitz
Journal:  Biochim Biophys Acta       Date:  1972-12-06

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Phosphorylation of initiation factor elF-2 and the control of reticulocyte protein synthesis.

Authors:  P J Farrell; K Balkow; T Hunt; R J Jackson; H Trachsel
Journal:  Cell       Date:  1977-05       Impact factor: 41.582

7.  Additional evidence that the hemin-controlled translational repressor from rabbit reticulocytes is a protein kinase.

Authors:  M Gross; J Mendelewski
Journal:  Biochem Biophys Res Commun       Date:  1977-01-24       Impact factor: 3.575

8.  Effect of hemin on site-specific phosphorylation of eukaryotic initiation factor 2.

Authors:  S M Tahara; J A Traugh; S B Sharp; T S Lundak; B Safer; W C Merrick
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

9.  Regulation of protein synthesis in reticulocyte lysates: phosphorylation of methionyl-tRNAf binding factor by protein kinase activity of translational inhibitor isolated from hemedeficient lysates.

Authors:  D Levin; R S Ranu; V Ernst; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205

10.  Specificity of the protein kinase activity associated with the hemin-controlled repressor of rabbit reticulocyte.

Authors:  G Kramer; J M Cimadevilla; B Hardesty
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205
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  7 in total

1.  Detection of activated double-stranded RNA-dependent protein kinase in 3T3-F442A cells.

Authors:  R Petryshyn; J J Chen; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

2.  Regulation of double-stranded RNA-activated eukaryotic initiation factor 2 alpha kinase by type 2 protein phosphatase in reticulocyte lysates.

Authors:  R Petryshyn; D H Levin; I M London
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

3.  Presence of haemin-controlled eIF-2 alpha kinases in both undifferentiated and differentiating mouse erythroleukaemia cells.

Authors:  T F Sarre
Journal:  Biochem J       Date:  1989-09-01       Impact factor: 3.857

4.  Protein synthesis in rabbit reticulocytes: characteristics of the protein factor RF that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates.

Authors:  M Grace; R O Ralston; A C Banerjee; N K Gupta
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

5.  Regulation of protein synthesis in vesicular stomatitis virus-infected mouse L-929 cells by decreased protein synthesis initiation factor 2 activity.

Authors:  M Centrella; J Lucas-Lenard
Journal:  J Virol       Date:  1982-03       Impact factor: 5.103

6.  Double-stranded RNA-dependent phosphorylation of protein P1 and eukaryotic initiation factor 2 alpha does not correlate with protein synthesis inhibition in a cell-free system from interferon-treated mouse L cells.

Authors:  H Jacobsen; D A Epstein; R M Friedmann; B Safer; P F Torrence
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

7.  Mode of action of the heme-controlled translational inhibitor: relationship of eukaryotic initiation factor 2-stimulating protein to translation restoring factor.

Authors:  J Siekierka; K I Mitsui; S Ochoa
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205
  7 in total

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