Literature DB >> 6932280

Purification of 20 alpha-hydroxysteroid dehydrogenase from human erythrocytes.

E Shirasawa, M Takeshita, Y Yoneyama.   

Abstract

20 alpha-hydroxysteroid dehydrogenase was found in the supernatant after hemolysis of human erythrocytes, and the enzyme was isolated from the membrane-free hemolysate on a DEAE-cellulose column. The NADPH-generating system was essential for the reaction.

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Year:  1980        PMID： 6932280     DOI： 10.1007/bf01976098

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  9 in total

1.  Extinction coefficients for use in equations for the spectrophotometric analysis of haemoglobin mixtures.

Authors:  O W van Assendelft; W G Zijlstra
Journal:  Anal Biochem       Date:  1975-11       Impact factor: 3.365

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Characterization of Enzyme-like activity of human hemoglobin. Properties of the hemoglobin-P-450 reductase-coupled aniline hydroxylase system.

Authors:  J J Mieyal; R S Ackerman; J L Blumer; L S Freeman
Journal:  J Biol Chem       Date:  1976-06-10       Impact factor: 5.157

4.  The influence of various factors upon testicular enzymes related to steroidogenesis.

Authors:  H Inano; H Nakano; M Shikita; B I Tamaoki
Journal:  Biochim Biophys Acta       Date:  1967-06-06

5.  Studies of the human testis. IV. Testicular 20 alpha-hydroxysteroid dehydrogenase and steroid 17 alpha-hydroxylase.

Authors:  D F Fan; H Oshima; B R Troen; P Troen
Journal:  Biochim Biophys Acta       Date:  1974-07-26

6.  Interconversion of progesterone and 20-alpha-dihydroprogesterone and of androstenedione and testosterone in vitro by blood and erythrocytes.

Authors:  H J van der Molen
Journal:  Acta Endocrinol (Copenh)       Date:  1968-07

7.  Progesterone metabolism in fetal blood.

Authors:  C D Nancarrow; R F Seamark
Journal:  Steroids       Date:  1968-09       Impact factor: 2.668

8.  The conversion of progesterone to delta 4 pregnene-20 alpha-ol, 3-one by human placenta in vitro.

Authors:  B LITTLE; J DIMARTINIS; B NYHOLM
Journal:  Acta Endocrinol (Copenh)       Date:  1959-04

9.  Isolation and characterization of 17 -hydroxy steroid dehydrogenase from human erythrocytes.

Authors:  E Mulder; G J Lamers-Stahlhofen; H J van der Molen
Journal:  Biochem J       Date:  1972-05       Impact factor: 3.857
  9 in total

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