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Literature DB >> 6929485

Three-dimensional crystals of membrane proteins: bacteriorhodopsin.

Abstract

The intrinsic membrane protein bacteriorhodopsin has been crystallized by salt precipitation after solubilization by octyl glucoside. Two different crystal forms were obtained, depending on the nature of the salt used and the pH. Needles formed in the presence of sodium phosphate and in ammonium sulfate solutions above pH 4.8. Cubes appeared in sodium citrate solutions or ammonium sulfate. Unlike the cubic crystals, the birefringent needles showed strong linear dichroism, which allowed determination of the orientation of the chromophore's transition moment. The procedure described here may be of general use in crystallographic studies of membrane proteins.

Entities: Chemical

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Substances：

Year: 1980 PMID： 6929485 PMCID： PMC348478 DOI： 10.1073/pnas.77.3.1283

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

9 in total

1. Bacteriorhodospin: a trans-membrane pump containing alpha-helix.

Authors: A E Blaurock
Journal: J Mol Biol Date: 1975-04-05 Impact factor: 5.469

2. Membrane crystals of ubiquinone:cytochrome c reductase from Neurospora mitochondria.

Authors: P Wingfield; T Arad; K Leonard; H Weiss
Journal: Nature Date: 1979-08-23 Impact factor: 49.962

3. Molecular structure determination by electron microscopy of unstained crystalline specimens.

Authors: P N Unwin; R Henderson
Journal: J Mol Biol Date: 1975-05-25 Impact factor: 5.469

4. The structure of the purple membrane from Halobacterium hallobium: analysis of the X-ray diffraction pattern.

Authors: R Henderson
Journal: J Mol Biol Date: 1975-04-05 Impact factor: 5.469

5. Formation and properties of bacteriorhodopsin monomers in the non-ionic detergents octyl-beta-D-glucoside and Triton X-100.

Authors: N A Dencher; M P Heyn
Journal: FEBS Lett Date: 1978-12-15 Impact factor: 4.124

6. Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane.

Authors: D Oesterhelt; W Stoeckenius
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

7. Biological membrane structure. 3. The lattice structure of membranous cytochrome oxidase.

Authors: G Vanderkooi; A E Senior; R A Capaldi; H Hayashi
Journal: Biochim Biophys Acta Date: 1972-07-03

8. Orthorhombic two-dimensional crystal form of purple membrane.

Authors: H Michel; D Oesterhelt; R Henderson
Journal: Proc Natl Acad Sci U S A Date: 1980-01 Impact factor: 11.205

9. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver.

Authors: J P Revel; M J Karnovsky
Journal: J Cell Biol Date: 1967-06 Impact factor: 10.539

9 in total

37 in total

1. Three-dimensional crystallization of the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily.

Authors: M Joanne Lemieux; Jinmei Song; Myong Jin Kim; Yafei Huang; Anthony Villa; Manfred Auer; Xiao-Dan Li; Da-Neng Wang
Journal: Protein Sci Date: 2003-12 Impact factor: 6.725

Review 5. The mystery of oxygen evolution: analysis of structure and function of photosystem II, the water-plastoquinone oxido-reductase.

Authors: M K Raval; B Biswal; U C Biswal
Journal: Photosynth Res Date: 2005-09 Impact factor: 3.573

Three-dimensional crystals of membrane proteins: bacteriorhodopsin.

1. Bacteriorhodospin: a trans-membrane pump containing alpha-helix.

2. Membrane crystals of ubiquinone:cytochrome c reductase from Neurospora mitochondria.

3. Molecular structure determination by electron microscopy of unstained crystalline specimens.

4. The structure of the purple membrane from Halobacterium hallobium: analysis of the X-ray diffraction pattern.

5. Formation and properties of bacteriorhodopsin monomers in the non-ionic detergents octyl-beta-D-glucoside and Triton X-100.

6. Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane.

7. Biological membrane structure. 3. The lattice structure of membranous cytochrome oxidase.

8. Orthorhombic two-dimensional crystal form of purple membrane.

9. Hexagonal array of subunits in intercellular junctions of the mouse heart and liver.

1. Three-dimensional crystallization of the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily.

2. Lipophilic mediated assays for beta-hematin inhibitors.

Review 3. Membrane protein crystallization in amphiphile phases: practical and theoretical considerations.

4. Research on photosynthetic reaction centers from 1932 to 1987.

Review 5. The mystery of oxygen evolution: analysis of structure and function of photosystem II, the water-plastoquinone oxido-reductase.

6. Frequencies of hydrophobic and hydrophilic runs and alternations in proteins of known structure.

7. My daily constitutional in martinsried.

Review 8. Strategies in the reassembly of membrane proteins into lipid bilayer systems and their functional assay.

9. George Feher: a pioneer in reaction center research.

Review 10. Binding energy, conformational change, and the mechanism of transmembrane solute movements.