Biogenesis of epithelial cell plasma membranes.

Polarized monolayers of cultured epithelial cells, such as the kidney-derived MDCK cell line, when infected with enveloped viruses, provide a convenient model system for study of the intracellular routes followed by newly synthesized glycoproteins to reach specific domains of the plasma membrane. The polarized nature of the monolayers is reflected in the asymmetric assembly of enveloped viruses, some of which, such as influenza and simian virus 5 (SV5), bud from the apical surfaces of the cells, while others, such as vesicular stomatitis virus (VSV), emerge from the basolateral surfaces. MDCK cells can sustain double infection with viruses of different budding polarity, and within such cells the envelope glycoproteins of the two viruses are synthesized simultaneously and assembled into virions at different sites. Immunoelectron microscopic observations of doubly infected cells show that glycoproteins of influenza and VSV traverse the same Golgi apparatus. This indicates that critical sorting steps must take place during or after passage of the glycoproteins through the organelle. Following passage through the Golgi, the HA glycoprotein accumulates almost exclusively at the apical surface, where the influenza virions assemble. Significant amounts of the G protein, however, are detected on both plasma membranes in singly and doubly infected cells, although VSV virion assembly is limited to basolateral domains. These observations indicate that the site of VSV budding is not exclusively determined by the presence of G polypeptides on a given cell-surface domain. It is possible that other cellular or viral components are responsible for the selection of the appropriate budding domain or that the G protein found on the apical surface must be transferred to the basolateral domain before it becomes competent for assembly.