Literature DB >> 6894794

A simple procedure for the isolation of brain actin.

M F Ruscha, R H Himes.   

Abstract

Actin has been isolated from bovine brain by forming an actin-containing gel from acetone powders. Solubilization of the gel and chromatography on Sephadex G150 produces actin in good yield. Acetone powders of crude pellets of brain homogenates obtained in the isolation of tubulin produces about 80% of the actin isolated from acetone powder of whole brain. The procedure therefore can be used to isolate both proteins from the same brain.

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Year:  1981        PMID: 6894794     DOI: 10.1080/00327488108061774

Source DB:  PubMed          Journal:  Prep Biochem        ISSN: 0032-7484


  3 in total

1.  Calpain II activity and calpastatin content in brain regions of 3- and 24-month-old rats.

Authors:  A Kenessey; M Banay-Schwartz; T DeGuzman; A Lajtha
Journal:  Neurochem Res       Date:  1990-03       Impact factor: 3.996

2.  Evidence for tubulin-binding sites on cellular membranes: plasma membranes, mitochondrial membranes, and secretory granule membranes.

Authors:  F Bernier-Valentin; D Aunis; B Rousset
Journal:  J Cell Biol       Date:  1983-07       Impact factor: 10.539

3.  Identical distribution of fluorescently labeled brain and muscle actins in living cardiac fibroblasts and myocytes.

Authors:  N McKenna; J B Meigs; Y L Wang
Journal:  J Cell Biol       Date:  1985-01       Impact factor: 10.539

  3 in total

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