Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Purification from starfish eggs of a protein that depolymerizes actin.

Literature DB >> 6894753

Purification from starfish eggs of a protein that depolymerizes actin.

Abstract

A protein which is capable of depolymerizing F-actin was purified from an extract of unfertilized starfish eggs by the use of DEAE-cellulose column chromatography and hydroxylapatite column chromatography. This protein has an apparent molecular weight of 17,000. It inhibited the extent of actin polymerization as well as depolymerizing F-actin rapidly. It was shown that this protein reacts with actin at a molar ratio of 1 : 1. The properties of this protein were compared with those of profilin from mammalian tissues.

Entities: Chemical Species

Mesh：

Substances：
Actins
Egg Proteins

Year: 1981 PMID： 6894753

Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387

Keyword Cloud
Cited

8 in total

1. F-actin and G-actin binding are uncoupled by mutation of conserved tyrosine residues in maize actin depolymerizing factor (ZmADF).

Authors: C J Jiang; A G Weeds; S Khan; P J Hussey
Journal: Proc Natl Acad Sci U S A Date: 1997-09-02 Impact factor: 11.205

2. Pollen specific expression of maize genes encoding actin depolymerizing factor-like proteins.

Authors: I Lopez; R G Anthony; S K Maciver; C J Jiang; S Khan; A G Weeds; P J Hussey
Journal: Proc Natl Acad Sci U S A Date: 1996-07-09 Impact factor: 11.205

3. A Zea mays pollen cDNA encoding a putative actin-depolymerizing factor.

Authors: M Rozycka; S Khan; I Lopez; A J Greenland; P J Hussey
Journal: Plant Physiol Date: 1995-03 Impact factor: 8.340

4. Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo.

Authors: Nobuhisa Umeki; Jun Nakajima; Taro Q P Noguchi; Kiyotaka Tokuraku; Akira Nagasaki; Kohji Ito; Keiko Hirose; Taro Q P Uyeda
Journal: J Biol Chem Date: 2012-12-03 Impact factor: 5.157

Purification from starfish eggs of a protein that depolymerizes actin.

1. F-actin and G-actin binding are uncoupled by mutation of conserved tyrosine residues in maize actin depolymerizing factor (ZmADF).

2. Pollen specific expression of maize genes encoding actin depolymerizing factor-like proteins.

3. A Zea mays pollen cDNA encoding a putative actin-depolymerizing factor.

4. Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo.

5. The changes in structural organization of actin in the sea urchin egg cortex in response to hydrostatic pressure.

Review 6. The ADF/cofilin family: actin-remodeling proteins.

Review 7. The actin depolymerizing factor (ADF)/cofilin signaling pathway and DNA damage responses in cancer.

8. An actin-depolymerizing protein (depactin) from starfish oocytes: properties and interaction with actin.