Literature DB >> 6894300

Mechanism of action of cytochalasin: evidence that it binds to actin filament ends.

S S Brown, J A Spudich.   

Abstract

To test the idea that cytochalasin retards actin assembly by binding to filament ends, we have designed a new assay for cytochalasin binding in which the number of filament ends can be varied independently of the total actin concentration. Actin is reacted with polylysine-coated polystyrene beads to make filament ends (Brown and Spudich, 1979, J. Cell Biol. 80:499-504) and then reacted with [3H]cytochalasin B. We have found that cytochalasin B binds to beads in the presence of actin, and that the number of cytochalasin B binding sites can be varied as a function of the number of filament ends independent of the total actin concentration by varying the bead concentration.

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Year:  1981        PMID： 6894300      PMCID： PMC2112756          DOI： 10.1083/jcb.88.3.487

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  15 in total

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Authors:  J H Hartwig; T P Stossel
Journal:  J Mol Biol       Date:  1979-11-05       Impact factor: 5.469

3.  Spectrin/actin complex isolated from sheep erythrocytes accelerates actin polymerization by simple nucleation. Evidence for oligomeric actin in the erythrocyte cytoskeleton.

Authors:  S L Brenner; E D Korn
Journal:  J Biol Chem       Date:  1980-02-25       Impact factor: 5.157

4.  Mechanism of action of cytochalasin B on actin.

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5.  Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin.

Authors:  M D Flanagan; S Lin
Journal:  J Biol Chem       Date:  1980-02-10       Impact factor: 5.157

6.  Substoichiometric concentrations of cytochalasin D inhibit actin polymerization. Additional evidence for an F-actin treadmill.

Authors:  S L Brenner; E D Korn
Journal:  J Biol Chem       Date:  1979-10-25       Impact factor: 5.157

7.  Biochemical and structural characterization of actin from Dictyostelium discoideum.

Authors:  D G Uyemura; S S Brown; J A Spudich
Journal:  J Biol Chem       Date:  1978-12-25       Impact factor: 5.157

8.  ATP-driven steady-state exchange of monomeric and filamentous actin from Dictyostelium discoideum.

Authors:  P A Simpson; J A Spudich
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205

9.  Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation.

Authors:  D C Lin; K D Tobin; M Grumet; S Lin
Journal:  J Cell Biol       Date:  1980-02       Impact factor: 10.539

10.  Nucleation of polar actin filament assembly by a positively charged surface.

Authors:  S S Brown; J A Spudich
Journal:  J Cell Biol       Date:  1979-02       Impact factor: 10.539
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  67 in total

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6.  Preparation of bead-tailed actin filaments: estimation of the torque produced by the sliding force in an in vitro motility assay.

Authors:  N Suzuki; H Miyata; S Ishiwata; K Kinosita
Journal:  Biophys J       Date:  1996-01       Impact factor: 4.033

7.  Ooplasmic segregation in theTubifex egg: Mode of pole plasm accumulation and possible involvement of microfilaments.

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Journal:  Wilehm Roux Arch Dev Biol       Date:  1982-07

8.  Early embryonic development of the dipteran insect Heteropeza pygmaea in the presence of cytoskeleton-affecting drugs.

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9.  Use of electric cell-substrate impedance sensing to assess in vitro cytotoxicity.

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Journal:  Biosens Bioelectron       Date:  2009-01-23       Impact factor: 10.618

10.  Reorganization of the endoplasmic reticulum in epidermal cells of onion bulb scales after cold stress: Involvement of cytoskeletal elements.

Authors:  H Quader; A Hofmann; E Schnepf
Journal:  Planta       Date:  1989-02       Impact factor: 4.116
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