Literature DB >> 6892925

An improved technique for separation of neutral glycosphingolipids by high-performance liquid chromatography.

A Suzuki, S K Kundu, D M Marcus.   

Abstract

We have developed a high-performance liquid chromatographic (HPLC) procedure for separation of O-acetyl-N-p-nitrobenzoyl derivatives of six neutral glycosphingolipids: glucosylceramide, lactosylceramide, globotriaosylceramide, lactotriaosylceramide, globotetraosylceramide, and neolactotetraosylceramide. The recoveries of glucosylceramide and globotetraosylceramide for the derivatization procedure and HPLC analysis were approximately 75%, and one nanomole of glycolipid could be detected. The procedure was used for analysis of human erythrocyte neutral glycolipids.

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Year:  1980        PMID: 6892925

Source DB:  PubMed          Journal:  J Lipid Res        ISSN: 0022-2275            Impact factor:   5.922


  8 in total

Review 1.  Sphingolipidomics: methods for the comprehensive analysis of sphingolipids.

Authors:  Christopher A Haynes; Jeremy C Allegood; Hyejung Park; M Cameron Sullards
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2008-12-31       Impact factor: 3.205

2.  Lactosylceramide in lysosomal storage disorders: a comparative immunohistochemical and biochemical study.

Authors:  H Hůlková; J Ledvinová; B Asfaw; K Koubek; K Kopriva; M Elleder
Journal:  Virchows Arch       Date:  2005-05-26       Impact factor: 4.064

3.  Dexamethasone-induced alterations in the glycosphingolipids of rat kidney.

Authors:  R Dahiya; T A Brasitus
Journal:  Lipids       Date:  1988-09       Impact factor: 1.880

4.  Premalignant alterations in the glycosphingolipids of small intestinal mucosa of rats treated with 1,2-dimethylhydrazine.

Authors:  R Dahiya; P K Dudeja; T A Brasitus
Journal:  Lipids       Date:  1988-05       Impact factor: 1.880

5.  Accumulation of globotriaosylceramide in a case of leiomyosarcoma.

Authors:  S C Li; S K Kundu; R Degasperi; Y T Li
Journal:  Biochem J       Date:  1986-12-15       Impact factor: 3.857

6.  Distribution of glycosphingolipids and ceramide of rat small intestinal mucosa.

Authors:  R Dahiya; T A Brasitus
Journal:  Lipids       Date:  1986-02       Impact factor: 1.880

7.  Distribution of glycosphingolipids of monkey small and large intestinal mucosa.

Authors:  R Dahiya; M D Brown; T A Brasitus
Journal:  Lipids       Date:  1986-02       Impact factor: 1.880

8.  Glycosphingolipids of fetal and adult sheep colonic mucosa.

Authors:  R Dahiya; T A Brasitus
Journal:  Lipids       Date:  1985-06       Impact factor: 1.880

  8 in total

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