Literature DB >> 689124

Suppression of CFU-s activity by rabbit-anti-mouse brain serum can be overcome by treatment with papain.

G van den Engh, M Platenburg.   

Abstract

The suppression of CFU-s activity which is observed after incubation with rabbit-anti-mouse brain sera (RAMB) can be reversed by incubating the antibody coated cells with papain. Papain treatment does not alter the CFU-s membrane since pre-treatment with the enzyme does not protect CFU-s from the lethal effect of RAMB. This technique should allow the use of fluoresceinated or otherwise labelled RAMB for the selection of CFU-s.

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Year:  1978        PMID: 689124

Source DB:  PubMed          Journal:  Exp Hematol        ISSN: 0301-472X            Impact factor:   3.084


  1 in total

1.  Antiserum to mouse pluripotent hemopoietic stem cells (CFU-S): evidence for a role of complement in the inactivation of CFU-S in diffusion chambers.

Authors:  S S Adler; R D Kuznetsky; F E Trobaugh
Journal:  Blut       Date:  1979-07
  1 in total

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