Literature DB >> 6888186

Primary cultures of bovine chromaffin cells synthesize and secrete vasoactive intestinal polypeptide (VIP).

L E Eiden, R L Eskay, J Scott, H Pollard, A J Hotchkiss.   

Abstract

Dispersed cells of the bovine adrenal medulla express immunoreactive vasoactive intestinal polypeptide (VIP) after 24 hours in culture, although VIP could not be detected in extracts of bovine adrenal medulla or cortex. Immunoreactive VIP eluted from a reversed-phrase chromatography column with the same retention time as authentic porcine VIP1-28. VIP in chromaffin cells in culture appears to be contained in a secretory granule pool, since it, like methionine-enkephalin (met-enk) was released into the medium after exposure of cells to nicotine, carbachol, veratridine and elevated potassium in a dose-dependent manner. Dose-response curves for VIP and enkephalin release by the above secretagogues were similar but not identical. Enkephalins and VIP may either be contained in separate subpopulations of chromaffin cells or co-stored in the same cells.

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Year:  1983        PMID： 6888186     DOI： 10.1016/0024-3205(83)90772-5

Source DB:  PubMed          Journal:  Life Sci        ISSN： 0024-3205            Impact factor:   5.037


  8 in total

Review 1.  Role of neurotransmitters and neuropeptides in the regulation of the adrenal cortex.

Authors:  C Delarue; V Contesse; S Lenglet; F Sicard; V Perraudin; H Lefebvre; M Kodjo; F Leboulenger; L Yon; N Gallo-Payet; H Vaudry
Journal:  Rev Endocr Metab Disord       Date:  2001-08       Impact factor: 6.514

2.  Immunohistochemical localization of oxytocin and vasopressin in the adrenal glands of rat, cow, hamster and guinea pig.

Authors:  J Hawthorn; S S Nussey; J R Henderson; J S Jenkins
Journal:  Cell Tissue Res       Date:  1987-10       Impact factor: 5.249

3.  Immunochemical characterization of carboxypeptidase B-like peptide-hormone-processing enzyme.

Authors:  V Y Hook; E Mezey; L D Fricker; R M Pruss; R E Siegel; M J Brownstein
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

Review 4.  Vasoactive intestinal peptide.

Authors:  S I Said
Journal:  J Endocrinol Invest       Date:  1986-04       Impact factor: 4.256

5.  Changes in adrenal neuropeptides content [peptide 7B2, neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP)] induced by pharmacological and hormonal manipulations.

Authors:  T Tsuchiya; Y Suzuki; H Suzuki; R Ohtake; S I Shimoda
Journal:  J Endocrinol Invest       Date:  1990-05       Impact factor: 4.256

6.  Processing of chromogranins in chromaffin cell culture: effects of reserpine and alpha-methyl-p-tyrosine.

Authors:  M Wolkersdorfer; A Laslop; C Lazure; R Fischer-Colbrie; H Winkler
Journal:  Biochem J       Date:  1996-06-15       Impact factor: 3.857

7.  Alternative modes of enkephalin biosynthesis regulation by reserpine and cyclic AMP in cultured chromaffin cells.

Authors:  L E Eiden; P Giraud; H U Affolter; E Herbert; A J Hotchkiss
Journal:  Proc Natl Acad Sci U S A       Date:  1984-07       Impact factor: 11.205

8.  Lineage-specific regulation of the vasoactive intestinal peptide gene in neuroblastoma cells is conferred by 5.2 kilobases of 5'-flanking sequence.

Authors:  J A Waschek; C M Hsu; L E Eiden
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205
  8 in total

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