Effects of various steroids on in vitro lifespan and cell growth of human fetal lung fibroblasts (WI-38).

Human fetal lung fibroblasts, WI-38, were cultivated in a medium containing various steroids. A dose-response curve constructed by counting the number of cells on day 16, or at regular intervals, showed that glucocorticoids (hydrocortisone and cortisone) and mineralocorticoids (aldosterone and deoxycorticosterone) caused an increase in cell density when added at physiological concentrations or higher. On the other hand, androgens (testosterone, dihydrotestosterone, and dehydroepiandrosterone), estrogen (17 beta-estradiol), and progesterone caused a decrease in cell density at higher concentrations (5 micrograms/ml, or more) although these had no effect on cell density at concentrations lower than 0.5 micrograms/ml. With cells grown from population doubling 31 in a medium containing steroids, it was shown that hydrocortisone extended the in vitro lifespan of WI-38 cells at concentrations of both 5 micrograms/ml and 0.5 micrograms/ml, while dehydroepiandrosterone, 17 beta-estradiol, and progesterone caused a shortening in lifespan at only 5 micrograms/ml. These results suggest that there is a direct relationship between the effects of steroids on cell growth and the lifespan of human fetal lung fibroblasts.