Literature DB >> 68832

Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.

H P Beck.   

Abstract

Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.

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Year:  1977        PMID: 68832     DOI: 10.1111/j.1365-2184.1977.tb00294.x

Source DB:  PubMed          Journal:  Cell Tissue Kinet        ISSN: 0008-8730


  3 in total

1.  Polyamine depletion increases cellular ribonucleotide levels.

Authors:  S M Oredsson; M Kanje; P S Mamont; J Wagner; O Heby
Journal:  Mol Cell Biochem       Date:  1986-04       Impact factor: 3.396

2.  Flow cytometry (FCM) of human epidermal cells. A preparation method for epidermal cells and demonstration of circadian variations in the proportion of S-phase cells.

Authors:  E Thorud; G Volden
Journal:  Arch Dermatol Res       Date:  1980       Impact factor: 3.017

3.  Polyamine starvation prolongs the S and G2 phases of polyamine-dependent (arginase-deficient) CHO cells.

Authors:  S Anehus; P Pohjanpelto; B Baldetorp; E Långström; O Heby
Journal:  Mol Cell Biol       Date:  1984-05       Impact factor: 4.272

  3 in total

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