| Literature DB >> 68832 |
Abstract
Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.Mesh:
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Year: 1977 PMID: 68832 DOI: 10.1111/j.1365-2184.1977.tb00294.x
Source DB: PubMed Journal: Cell Tissue Kinet ISSN: 0008-8730