Literature DB >> 6882371

Measurement of specific radioactivities in labelled hormones by self-displacement analysis.

J C Calvo, J P Radicella, E H Charreau.   

Abstract

A graphical method is described that allows the determination of specific radioactivities of radioactively labelled hormones. This method combines the self-displacement technique, plotting bound/free ratios versus mass of unlabelled hormone or total radioactivity of labelled preparation added to the receptor preparation, and the maximal binding capacity of the labelled hormone. The procedure presented herein provides a more realistic specific radioactivity for use in all binding experiments. Application of the method is demonstrated for 125I-labelled ovine prolactin, and data are presented for 125I-labelled human choriogonadotropin and [3H]testosterone.

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Year:  1983        PMID： 6882371      PMCID： PMC1152042          DOI： 10.1042/bj2120259

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  Gonadotroping binding and activation of the interstitial cells of the testis.

Authors:  K J Catt; T Tsuruhara; C Mendelson; J M Ketelslegers; M L Dufau
Journal:  Curr Top Mol Endocrinol       Date:  1974

2.  hCG-Leydig cell functional binding kinetics: threshold and nonlinearity in response.

Authors:  W R Moyle; M Netburn; A E Cosgrove; J Krieger; O P Bahl
Journal:  Am J Physiol       Date:  1980-03

3.  Gonadotropin binding and stimulation of cyclic adenosine 3':5'-monophosphate and testosterone production in isolated Leydig cells.

Authors:  C Mendelson; M Dufau; K Catt
Journal:  J Biol Chem       Date:  1975-11-25       Impact factor: 5.157

4.  Enzymatic iodination of polypeptides with 125I to high specific activity.

Authors:  J I Thorell; B G Johansson
Journal:  Biochim Biophys Acta       Date:  1971-12-28

5.  Preparation of biologically active mono-125I-insulin of high specific activity.

Authors:  J C Cresto; D P Udrisar; M C Camberos; J C Basabe; P Gómez Acuña; S F de Majo
Journal:  Acta Physiol Lat Am       Date:  1981
  5 in total
  33 in total

1.  Mechanism of kinase activation in the receptor for colony-stimulating factor 1.

Authors:  A W Lee; A W Nienhuis
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

2.  Endothelin binding to cultured calf adrenal zona glomerulosa cells and stimulation of aldosterone secretion.

Authors:  E N Cozza; C E Gomez-Sanchez; M F Foecking; S Chiou
Journal:  J Clin Invest       Date:  1989-09       Impact factor: 14.808

3.  In vitro-derived leukemic erythroid cell lines induced by a raf- and myc-containing retrovirus differentiate in response to erythropoietin.

Authors:  S P Klinken; N A Nicola; G R Johnson
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

4.  Effects of recombinant tumor necrosis factor on proliferation and differentiation of leukemic and normal hemopoietic cells in vitro. Relationship to cell surface receptor.

Authors:  C Peetre; U Gullberg; E Nilsson; I Olsson
Journal:  J Clin Invest       Date:  1986-12       Impact factor: 14.808

Review 5.  Interferon receptors.

Authors:  A A Branca
Journal:  In Vitro Cell Dev Biol       Date:  1988-03

6.  Reciprocal inhibition of binding between interleukin 3 and granulocyte-macrophage colony-stimulating factor to human eosinophils.

Authors:  A F Lopez; J M Eglinton; D Gillis; L S Park; S Clark; M A Vadas
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

7.  Changes in oestrogen, progesterone and epidermal growth factor receptor concentrations and affinities during the oestrous cycle in the normal mammary gland and uterus of dogs.

Authors:  I Donnay; P Wouters-Ballman; N Devleeschouwer; G Leclercq; J Verstegen
Journal:  Vet Res Commun       Date:  1995       Impact factor: 2.459

8.  Interleukin-6 stimulates cell proliferation of rat pituitary clonal cell lines in vitro.

Authors:  T Sawada; K Koike; Y Kanda; H Ikegami; H Jikihara; T Maeda; Y Osako; K Hirota; A Miyake
Journal:  J Endocrinol Invest       Date:  1995-02       Impact factor: 4.256

9.  Binding of the differentiation-inducer, granulocyte-colony-stimulating factor, to responsive but not unresponsive leukemic cell lines.

Authors:  N A Nicola; D Metcalf
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

10.  Structure-function relationships of interleukin-3. An analysis based on the function and binding characteristics of a series of interspecies chimera of gibbon and murine interleukin-3.

Authors:  K Kaushansky; S G Shoemaker; V C Broudy; N L Lin; J V Matous; E M Alderman; J D Aghajanian; P J Szklut; R E VanDyke; M K Pearce
Journal:  J Clin Invest       Date:  1992-11       Impact factor: 14.808
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