Literature DB >> 6882366

Purification and characterization of an antibacterial protein from haemolymph of Sarcophaga peregrina (flesh-fly) larvae.

M Okada, S Natori.   

Abstract

Three antibacterial proteins were induced when the body wall of Sarcophaga peregrina (flesh-fly) larvae was injured with a hypodermic needle. These proteins were separated and one was purified to homogeneity. The molecular weight of the purified protein was 5000 and its amino acid composition was similar to that of cecropins, which are antibacterial proteins in Hyalophora cecropia (cecropia moth) pupae. This protein was found to have bactericidal activity and to be effective at a concentration of 0.1 micrograms/ml against certain Gram-negative and Gram-positive bacteria.

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Year:  1983        PMID: 6882366      PMCID: PMC1154420          DOI: 10.1042/bj2110727

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  15 in total

1.  Insect immunity. III. Purification and partial characterization of immune protein P5 from hemolymph of Hyalophora cecropia pupae.

Authors:  A E Pye; H G Boman
Journal:  Infect Immun       Date:  1977-08       Impact factor: 3.441

2.  The encapsulation of foreign tissue implants in Galleria mellonella larvae.

Authors:  A R Schmit; N A Ratcliffe
Journal:  J Insect Physiol       Date:  1977       Impact factor: 2.354

3.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

4.  Recognition of foreignness in invertebrates. II. In vitro studies of cockroach phagocytic haemocytes.

Authors:  M T Scott
Journal:  Immunology       Date:  1971-11       Impact factor: 7.397

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  A possible mechanism of induction of insect lectin.

Authors:  H Komano; D Mizuno; S Natori
Journal:  J Biol Chem       Date:  1981-07-25       Impact factor: 5.157

7.  Purification of lectin induced in the hemolymph of Sarcophaga peregrina larvae on injury.

Authors:  H Komano; D Mizuno; S Natori
Journal:  J Biol Chem       Date:  1980-04-10       Impact factor: 5.157

8.  Insect immunity. Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia.

Authors:  D Hultmark; H Steiner; T Rasmuson; H G Boman
Journal:  Eur J Biochem       Date:  1980-05

9.  Sequence and specificity of two antibacterial proteins involved in insect immunity.

Authors:  H Steiner; D Hultmark; A Engström; H Bennich; H G Boman
Journal:  Nature       Date:  1981-07-16       Impact factor: 49.962

10.  Insect immunity. I. Characteristics of an inducible cell-free antibacterial reaction in hemolymph of Samia cynthia pupae.

Authors:  H G Boman; I Nilsson-Faye; K Paul; T Rasmuson
Journal:  Infect Immun       Date:  1974-07       Impact factor: 3.441

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  24 in total

1.  Silkworm apolipophorin protein inhibits Staphylococcus aureus virulence.

Authors:  Yuichi Hanada; Kazuhisa Sekimizu; Chikara Kaito
Journal:  J Biol Chem       Date:  2011-09-20       Impact factor: 5.157

2.  Analysis of a gene cluster for sarcotoxin II, a group of antibacterial proteins of Sarcophaga peregrina.

Authors:  A Kanai; S Natori
Journal:  Mol Cell Biol       Date:  1990-12       Impact factor: 4.272

3.  Recognition and elimination of diversified pathogens in insect defense systems.

Authors:  Shoichiro Kurata
Journal:  Mol Divers       Date:  2006-11       Impact factor: 2.943

4.  Production of recombinant sarcotoxin IA in Bombyx mori cells.

Authors:  K Yamada; Y Nakajima; S Natori
Journal:  Biochem J       Date:  1990-12-15       Impact factor: 3.857

5.  Antibacterial activity of selected invertebrate species.

Authors:  Salwa Mansur Ali; Ruqaiyayh Siddiqui; Kuppusamy A Sagathevan; Naveed Ahmed Khan
Journal:  Folia Microbiol (Praha)       Date:  2021-03-11       Impact factor: 2.099

6.  Insect cytokine paralytic peptide (PP) induces cellular and humoral immune responses in the silkworm Bombyx mori.

Authors:  Kenichi Ishii; Hiroshi Hamamoto; Manabu Kamimura; Yuki Nakamura; Hiroaki Noda; Katsutoshi Imamura; Kazuei Mita; Kazuhisa Sekimizu
Journal:  J Biol Chem       Date:  2010-07-09       Impact factor: 5.157

7.  Ionophore activity of sarcotoxin I, a bactericidal protein of Sarcophaga peregrina.

Authors:  M Okada; S Natori
Journal:  Biochem J       Date:  1985-07-15       Impact factor: 3.857

8.  Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kappa B-binding motifs of the defense protein genes of Sarcophaga peregrina (the flesh fly).

Authors:  A Kobayashi; M Matsui; T Kubo; S Natori
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272

9.  Prevention of death in bacterium-infected mice by a synthetic antimicrobial peptide, L5, through activation of host immunity.

Authors:  Yukiko Okuyama-Nishida; Nobuko Akiyama; Giichi Sugimori; Kazuhide Nomura; Kenji Ogawa; Koichi J Homma; Kazuhisa Sekimizu; Masafumi Tsujimoto; Shunji Natori
Journal:  Antimicrob Agents Chemother       Date:  2009-03-16       Impact factor: 5.191

10.  A theoretical approach to spot active regions in antimicrobial proteins.

Authors:  Marc Torrent; Victòria M Nogués; Ester Boix
Journal:  BMC Bioinformatics       Date:  2009-11-11       Impact factor: 3.169

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