Selective medium for the isolation of Mycoplasma bovis from nasal discharges of pneumonic calves.

Strains of bovine Mycoplasmatales exhibited two growth forms on a plate medium containing 0.1 per cent Tween 80. Colonies of Mycoplasma bovis could easily be differentiated from those of concomitant mycoplasmatales in that the former grew rapidly with film and spots, while colonies of M arginini and Acholeplasma species did not produce film and spots; the growth of M bovirhinis was completely inhibited.