Literature DB >> 6875261

Direct measurement of in vitro antibody production using an enzyme-linked immunosorbent assay.

G H Boerrigter, A Vos, R J Scheper.   

Abstract

A rapid and sensitive enzyme-linked immunoassay is described for quantitation of the secretion of cellular products during short-term (1-20 h) culture in vitro. The method is based on culture of cells, serially diluted in antigen or antibody-coated polystyrene microtitre wells. The wide applicability of this method for measurement of anti-hapten antibody, immunoglobulin and lymphokine production is stressed.

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Year:  1983        PMID: 6875261     DOI: 10.1016/0022-1759(83)90234-x

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN: 0022-1759            Impact factor:   2.303


  1 in total

1.  Lymph node cells from rats with Heymann's nephritis produce in vitro autoantibodies directed against purified renal tubular antigen.

Authors:  E de Heer; M R Daha; L A van Es
Journal:  Immunology       Date:  1984-08       Impact factor: 7.397

  1 in total

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