Literature DB >> 6873295

In vivo 31P-NMR studies of myocardial high energy phosphate metabolism during anoxia and recovery.

K J Neurohr, G Gollin, E J Barrett, R G Shulman.   

Abstract

31P NMR spectra of heart in-situ in live guinea pigs were obtained continuously in 20.5 s time blocks during 3 min of anoxia, during subsequent reoxygenation and, in separate animals, during terminal anoxia. Reversible anoxia resulted in rapid degradation of phosphocreatine (t 1/2 = 54.5 +/- 2.5 s) which recovered fully during reoxygenation. Heart Pi increased during anoxia and returned to basal levels after oxygen was restored. During 3 min of anoxia, no significant changes in ATP levels or pH were detected. The results demonstrate that it is feasible to measure rapid fluxes of high energy phosphates by 31P NMR in intact animals during and after anoxic stress to the myocardium.

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Year:  1983        PMID: 6873295     DOI: 10.1016/0014-5793(83)80447-5

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  2 in total

Review 1.  Metabolic regulation of in vivo myocardial contractile function: multiparameter analysis.

Authors:  M D Osbakken
Journal:  Mol Cell Biochem       Date:  1994 Apr-May       Impact factor: 3.396

2.  Noninvasive detection and monitoring of regional myocardial ischemia in situ using depth-resolved 31P NMR spectroscopy.

Authors:  P A Bottomley; R J Herfkens; L S Smith; S Brazzamano; R Blinder; L W Hedlund; J L Swain; R W Redington
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

  2 in total

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