Literature DB >> 6873241

Purification of diphtheria toxin by chromatography on Cibacron Blue-Sepharose.

G Antoni, M Bigio, G Borri, M C Casagli, P Neri.   

Abstract

Diphtheria toxin binds to Cibacron Blue-Agarose and may be eluted by increasing the ionic strength of the elution buffer. Experiments using difference spectroscopy showed that the interaction between toxin and dye is ionic rather than hydrophobic, and therefore it is of a different nature with respect to that usually found in nucleotide-requiring enzymes.

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Year:  1983        PMID: 6873241     DOI: 10.1007/bf01990419

Source DB:  PubMed          Journal:  Experientia        ISSN: 0014-4754


  7 in total

Review 1.  Diphtheria toxin: mode of action and structure.

Authors:  R J Collier
Journal:  Bacteriol Rev       Date:  1975-03

2.  Affinity chromatography purification of diphtheria toxin.

Authors:  G Cukor; J D Readio; R J Kuchler
Journal:  Biotechnol Bioeng       Date:  1974-07       Impact factor: 4.530

3.  Structure and activity of diphtheria toxin. II. Attack by trypsin at a specific site within the intact toxin molecule.

Authors:  R Drazin; J Kandel; R J Collier
Journal:  J Biol Chem       Date:  1971-03-10       Impact factor: 5.157

4.  Different interactions of human and bovine serum albumin with Cibacron Blue and Blue Dextran.

Authors:  G Antoni; M C Casagli; M Bigio; G Borri; P Neri
Journal:  Ital J Biochem       Date:  1982 Mar-Apr

5.  Ligand interactions of diphtheria toxin. I. Binding and hydrolysis of NAD.

Authors:  S Lory; S F Carroll; P D Bernard; R J Collier
Journal:  J Biol Chem       Date:  1980-12-25       Impact factor: 5.157

6.  Dihydrofolate reductases from chicken liver and Lactobacillus casei bind Cibacron blue F3GA in different modes and at different sites.

Authors:  S Subramanian; B T Kaufman
Journal:  J Biol Chem       Date:  1980-11-25       Impact factor: 5.157

7.  Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies.

Authors:  C B Laurell
Journal:  Anal Biochem       Date:  1966-04       Impact factor: 3.365

  7 in total

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