Literature DB >> 6869826

A rapid method for removal of detergents from protein solution.

T Horigome, H Sugano.   

Abstract

A simple and rapid technique is described for the removal of Triton X-100, deoxycholate, and cholate from protein solutions. The method involves a 2-min centrifugation of the sample on a Bio-Beads SM-2 bed prepared in a microcentrifuge tube and is suitable for multiple assays of 0.05- to 0.45-ml samples. Another advantage of this method is the high recovery of proteins without dilution of the sample.

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Year:  1983        PMID： 6869826     DOI： 10.1016/0003-2697(83)90605-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  Change of substrate specificity of rat liver microsomal fatty acyl-CoA synthetase activity by Triton X-100.

Authors:  K Nagamatsu; S Soeda; Y Kishimoto
Journal:  Lipids       Date:  1986-05       Impact factor: 1.880

2.  Methodology for assaying iodide conductance in proteoliposomes: specific induction by thyroid membrane protein.

Authors:  P E Golstein; A Sener; R Beauwens
Journal:  Biochem J       Date:  1995-12-01       Impact factor: 3.857

3.  Association of Phosphatidylinositol 4-Kinase with the Plant Cytoskeleton.

Authors:  P. Xu; C. W. Lloyd; C. J. Staiger; B. K. Drobak
Journal:  Plant Cell       Date:  1992-08       Impact factor: 11.277

4.  A study of GDP binding to purified thermogenin protein from brown adipose tissue.

Authors:  R R French; M G Gore; D A York
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857

5.  Removal of bound Triton X-100 from purified bovine heart cytochrome bc1.

Authors:  Rastislav Varhac; Neal C Robinson; Andrej Musatov
Journal:  Anal Biochem       Date:  2009-09-03       Impact factor: 3.365

6.  Specificity of antibodies against Neisseria gonorrhoeae that stimulate neutrophil chemotaxis. Role of antibodies directed against lipooligosaccharides.

Authors:  P Densen; S Gulati; P A Rice
Journal:  J Clin Invest       Date:  1987-07       Impact factor: 14.808

7.  Purification and immunochemical characterization of a dihydrofolate reductase-thymidylate synthase enzyme complex from wild-carrot cells.

Authors:  I Toth; G Lazar; H M Goodman
Journal:  EMBO J       Date:  1987-07       Impact factor: 11.598

8.  Characterization of the junctional face membrane from terminal cisternae of sarcoplasmic reticulum.

Authors:  B Costello; C Chadwick; A Saito; A Chu; A Maurer; S Fleischer
Journal:  J Cell Biol       Date:  1986-09       Impact factor: 10.539

9.  Immunoglobulin G antibodies directed against protein III block killing of serum-resistant Neisseria gonorrhoeae by immune serum.

Authors:  P A Rice; H E Vayo; M R Tam; M S Blake
Journal:  J Exp Med       Date:  1986-11-01       Impact factor: 14.307
  9 in total

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