Literature DB >> 6869796

An improved cathepsin-D substrate and assay procedure.

N Agarwal, D H Rich.   

Abstract

Ten analogs of the peptide A-Phe(NO2)-Phe-Val-Leu-B were synthesized and tested as substrates for cathepsin D and pepsin. The best substrate found for cathepsin D, Phe-Ala-Ala-Phe(NO2)-Phe-Val-Leu-OM4P (kcat = 2.9 s-1; Km = 7.1 microM), has the largest kcat/Km value (408 mM-1 s-1) reported to date for this enzyme. The effect of peptide structure on solubility and kinetic parameters is discussed. The peptide provides a useful new substrate for continuous assay of cathepsin D.

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Year:  1983        PMID： 6869796     DOI： 10.1016/0003-2697(83)90663-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  5 in total

1.  A systematic series of synthetic chromophoric substrates for aspartic proteinases.

Authors:  B M Dunn; M Jimenez; B F Parten; M J Valler; C E Rolph; J Kay
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2.  A sensitive procedure for determination of cathepsin D: activity in alveolar and peritoneal macrophages.

Authors:  M Orlowski; R Orlowski; J C Chang; E Wilk; M Lesser
Journal:  Mol Cell Biochem       Date:  1984-09       Impact factor: 3.396

3.  SC-52151, a novel inhibitor of the human immunodeficiency virus protease.

Authors:  M Bryant; D Getman; M Smidt; J Marr; M Clare; R Dillard; D Lansky; G DeCrescenzo; R Heintz; K Houseman
Journal:  Antimicrob Agents Chemother       Date:  1995-10       Impact factor: 5.191

4.  A pepstatin-insensitive aspartic proteinase from a thermophilic Bacillus sp.

Authors:  H S Toogood; M Prescott; R M Daniel
Journal:  Biochem J       Date:  1995-05-01       Impact factor: 3.857

5.  A structural biology approach to understand human lymphatic filarial infection.

Authors:  Raghavendra Sashi Krishna Nagampalli; Krishnasamy Gunasekaran; Rangarajan Badri Narayanan; Angela Peters; Rajagopalan Bhaskaran
Journal:  PLoS Negl Trop Dis       Date:  2014-02-06
  5 in total

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