| Literature DB >> 686706 |
B K Lee, R G Condon, M Patel, E M Oden, J A Waitz.
Abstract
We have developed a qualitative method to assay for the presence of 2-deoxystreptamine in hydrolysates of crude aminoglycoside preparations using a 2-deoxystreptamine-requiring idiotrophic mutant. The assay involves (i) incubation of a 2-deoxystreptamine-requiring mutant with 1 mg of a hydrolyzed preparation from a crude unknown antibiotic mixture per ml, and (ii) examination of the resultant incubation mixture for production of antibiotic(s) by disk assay.Entities:
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Year: 1978 PMID: 686706 PMCID: PMC352421 DOI: 10.1128/AAC.14.1.157
Source DB: PubMed Journal: Antimicrob Agents Chemother ISSN: 0066-4804 Impact factor: 5.191