Literature DB >> 686706

Method for qualitative determination of 2-deoxystreptamine.

B K Lee, R G Condon, M Patel, E M Oden, J A Waitz.   

Abstract

We have developed a qualitative method to assay for the presence of 2-deoxystreptamine in hydrolysates of crude aminoglycoside preparations using a 2-deoxystreptamine-requiring idiotrophic mutant. The assay involves (i) incubation of a 2-deoxystreptamine-requiring mutant with 1 mg of a hydrolyzed preparation from a crude unknown antibiotic mixture per ml, and (ii) examination of the resultant incubation mixture for production of antibiotic(s) by disk assay.

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Year:  1978        PMID: 686706      PMCID: PMC352421          DOI: 10.1128/AAC.14.1.157

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


  3 in total

1.  Mutamicins; biosynthetically created new sisomicin analogues.

Authors:  R T Testa; G H Wagman; P J Daniels; M J Weinstein
Journal:  J Antibiot (Tokyo)       Date:  1974-12       Impact factor: 2.649

2.  Incorporation of L-methionine-methyl-14C into gentamicins.

Authors:  B K Lee; R T Testa; G H Wagman; C M Liu; L McDaniel; C Schaffner
Journal:  J Antibiot (Tokyo)       Date:  1973-12       Impact factor: 2.649

3.  Antibiotic 6640, a new Micromonospora-produced aminoglycoside antibiotic.

Authors:  M J Weinstein; J A Marquez; R T Testa; G H Wagman; E M Oden; J A Waitz
Journal:  J Antibiot (Tokyo)       Date:  1970-11       Impact factor: 2.649

  3 in total

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