Literature DB >> 6866765

Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction.

P Sloof, H H Menke, M P Caspers, P Borst.   

Abstract

We have size-fractionated intact DNA from Trypanosoma brucei into a major large DNA fraction (greater than 350S) and minor middle-sized (60-250S) and small (less than 60S) DNA fractions. Large DNA contains the rRNA genes, the basic copy genes for several variant surface glycoproteins (VSGs), including one which lies near a telomer, and the expression-linked copies of the two VSG genes. The middle-sized DNA contains at least one VSG gene, but the hybridization of this fraction with probes for the conserved repetitive sequences that mark the edges of the transposed segments of VSG genes, suggests that it may contain many VSG genes. The 177-bp repeat satellite DNA is also exclusively found in this fraction.

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Year:  1983        PMID： 6866765      PMCID： PMC326014          DOI： 10.1093/nar/11.12.3889

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  35 in total

Review 1.  The structure and isomerization of herpes simplex virus genomes.

Authors:  B Roizman
Journal:  Cell       Date:  1979-03       Impact factor: 41.582

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Human cytomegalovirus DNA. I. Molecular weight and infectivity.

Authors:  J L Geelen; C Walig; P Wertheim; J van der Noordaa
Journal:  J Virol       Date:  1978-06       Impact factor: 5.103

4.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

5.  Molecular weights of homogeneous coliphage DNA's from density-gradient sedimentation equilibrium.

Authors:  C W Schmid; J E Hearst
Journal:  J Mol Biol       Date:  1969-08-28       Impact factor: 5.469

6.  Chromosome structure in ciliated protozoans.

Authors:  D M Prescott; K G Murti
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1974

7.  A physical map of the DNA regions flanking the rabbit beta-globin gene.

Authors:  A J Jeffreys; R A Flavell
Journal:  Cell       Date:  1977-10       Impact factor: 41.582

8.  The organization of ribosomal RNA genes in the mitochondrial DNA of Tetrahymena pyriformis strain ST.

Authors:  R W Goldbach; P Borst; J E Bollen-de Boer; E F van Bruggen
Journal:  Biochim Biophys Acta       Date:  1978-11-21

9.  Spindle microtubules in the dividing nuclei of trypanosomes.

Authors:  K Vickerman; T M Preston
Journal:  J Cell Sci       Date:  1970-03       Impact factor: 5.285

10.  Isolation and characterization of kinetoplast DNA from bloodstream form of Trypanosoma brucei.

Authors:  A H Fairlamb; P O Weislogel; J H Hoeijmakers; P Borst
Journal:  J Cell Biol       Date:  1978-02       Impact factor: 10.539
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  20 in total

Review 1.  Repetitive elements in genomes of parasitic protozoa.

Authors:  Bill Wickstead; Klaus Ersfeld; Keith Gull
Journal:  Microbiol Mol Biol Rev       Date:  2003-09       Impact factor: 11.056

2.  The small chromosomes of Trypanosoma brucei involved in antigenic variation are constructed around repetitive palindromes.

Authors:  Bill Wickstead; Klaus Ersfeld; Keith Gull
Journal:  Genome Res       Date:  2004-06       Impact factor: 9.043

3.  Chromosome structure: DNA nucleotide sequence elements of a subset of the minichromosomes of the protozoan Trypanosoma brucei.

Authors:  M Weiden; Y N Osheim; A L Beyer; L H Van der Ploeg
Journal:  Mol Cell Biol       Date:  1991-08       Impact factor: 4.272

4.  The telomeric GGGTTA repeats of Trypanosoma brucei contain the hypermodified base J in both strands.

Authors:  F van Leeuwen; E R Wijsman; E Kuyl-Yeheskiely; G A van der Marel; J H van Boom; P Borst
Journal:  Nucleic Acids Res       Date:  1996-07-01       Impact factor: 16.971

Review 5.  Molecular biology of trypanosome antigenic variation.

Authors:  J E Donelson; A C Rice-Ficht
Journal:  Microbiol Rev       Date:  1985-06

6.  A novel DNA nucleotide in Trypanosoma brucei only present in the mammalian phase of the life-cycle.

Authors:  J Gommers-Ampt; J Lutgerink; P Borst
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

7.  Minichromosomal repetitive DNA in Trypanosoma cruzi: its use in a high-sensitivity parasite detection assay.

Authors:  A Gonzalez; E Prediger; M E Huecas; N Nogueira; P M Lizardi
Journal:  Proc Natl Acad Sci U S A       Date:  1984-06       Impact factor: 11.205

8.  The IsTat 1.3 VSG multigene family in Trypanosoma brucei: retention of the expression linked copy through multiple antigenic switches.

Authors:  J Allison; V Rothwell; G Newport; N Agabian; K Stuart
Journal:  Nucleic Acids Res       Date:  1984-12-11       Impact factor: 16.971

9.  Construction of trypanosome artificial mini-chromosomes.

Authors:  M G Lee; Y E; N Axelrod
Journal:  Nucleic Acids Res       Date:  1995-12-11       Impact factor: 16.971

10.  A ribosomal RNA gene promoter at the telomere of a mini-chromosome in Trypanosoma brucei.

Authors:  J C Zomerdijk; R Kieft; P Borst
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971
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