Literature DB >> 6866762

Polyribosome binding of rabbit globin messenger RNA and messenger ribonucleoprotein labelled with bacteriophage-T4 RNA ligase and 5'-[32P] phosphocytidine 3'-phosphate.

N S Thomas, P D Butcher, H R Arnstein.   

Abstract

Rabbit polyribosomal globin messenger RNA (mRNA) and messenger ribonucleoprotein (mRNP) were labelled at the 3' poly(A) tail to high specific activity with T4 RNA ligase and [5'-(32)P]pCp without consequent loss of functional activity. Labelled message was translated in both micrococcal nuclease treated and untreated rabbit reticulocyte lysates, as shown by the formation of labelled polyribosomes. The utilisation of labelled messenger was abolished by T2 toxin or sodium fluoride which are known to inhibit protein synthesis.Images

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Year:  1983        PMID： 6866762      PMCID： PMC325686     

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  31 in total

1.  Inhibition at the initiation level of eukaryotic protein synthesis by T-2 toxin.

Authors:  K E Smith; M Cannon; E Cundliffe
Journal:  FEBS Lett       Date:  1975-01-15       Impact factor: 4.124

2.  Synthesis of alpha- and beta-globin directed by messenger ribonucleoprotein from rabbit reticulocytes.

Authors:  V Ernst; H R Arnstein
Journal:  Biochim Biophys Acta       Date:  1975-01-20

3.  Fluoride inhibition of the initiation of protein synthesis in the reticulocyte lysate cell-free system.

Authors:  J C O'Rourke; W Godchaux
Journal:  J Biol Chem       Date:  1975-05-10       Impact factor: 5.157

4.  Initiator tRNA for the synthesis of globin peptides.

Authors:  W Culp; J Morrisey; B Hardesty
Journal:  Biochem Biophys Res Commun       Date:  1970-08-24       Impact factor: 3.575

5.  Scintillation counting of 32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic media.

Authors:  R T Haviland; L L Bieber
Journal:  Anal Biochem       Date:  1970-02       Impact factor: 3.365

6.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

7.  Occurrence of cysteinyl transfer ribonucleic acid with a blocked alpha-amino group in rabbit reticulocytes.

Authors:  J Pawelek; W Godchaux; J Grasso; A I Skoultchi; J Eisenstadt; P Lengyel
Journal:  Biochim Biophys Acta       Date:  1971-03-11

8.  Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver.

Authors:  J H Parish; K S Kirby
Journal:  Biochim Biophys Acta       Date:  1966-12-21

9.  Effect of aurintricarboxylic acid and of NaF on the binding of globin messenger RNA to reticulocyte 40S ribosomal subunits.

Authors:  B Lebleu; G Marbaix; J Wérenne; A Burny; G Huez
Journal:  Biochem Biophys Res Commun       Date:  1970-08-11       Impact factor: 3.575

10.  Initiation of protein synthesis in a rabbit reticulocyte lysate system.

Authors:  W Hoerz; K S McCarty
Journal:  Biochim Biophys Acta       Date:  1971-01-28
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  4 in total

Review 1.  Regulation of mRNA polyadenylation-deadenylation.

Authors:  C M Tsiapalis
Journal:  Mol Biol Rep       Date:  1987       Impact factor: 2.316

2.  Phosphorylation of a 60 kDa polypeptide from Xenopus oocytes blocks messenger RNA translation.

Authors:  D Kick; P Barrett; A Cummings; J Sommerville
Journal:  Nucleic Acids Res       Date:  1987-05-26       Impact factor: 16.971

3.  Covalent enzyme-RNA complex: a tRNA modification that prevents a covalent enzyme interaction also prevents aminoacylation.

Authors:  R Starzyk; H Schoemaker; P Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

4.  Computer-aided nucleic acid secondary structure modeling incorporating enzymatic digestion data.

Authors:  G J Quigley; L Gehrke; D A Roth; P E Auron
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971
  4 in total

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