Literature DB >> 6865930

The NADPH oxidase of guinea pig polymorphonuclear leucocytes. Properties of the deoxycholate extracted enzyme.

P Bellavite, M C Serra, A Davoli, J V Bannister, F Rossi.   

Abstract

NADPH oxidase from stimulated guinea pig granulocytes was extracted with deoxycholate. The solubilized enzyme was stable in 20% glycerol. Solubilized enzyme was free of myeloperoxidase activity. The properties of the deoxycholate solubilized enzyme indicated that it is a high molecular weight complex with a flavoprotein, calmodulin and cytochrome b possibly forming part of the complex. Maximum activity was between pH 7.0 and 7.5. The Km value was 15.8 microM for NADPH and 434 microM for NADH indicating that NADPH is the preferential substrate.

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Year:  1983        PMID： 6865930     DOI： 10.1007/bf00230585

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  29 in total

1.  CHANGES IN THE METABOLIC PATTERN OF POLYMORPHO-NUCLEAR LEUCOCYTES DURING PHAGOCYTOSIS.

Authors:  F ROSSI; M ZATTI
Journal:  Br J Exp Pathol       Date:  1964-10

2.  Enzymatic basis of metabolic stimulation in leucocytes during phagocytosis: the role of activated NADPH oxidase.

Authors:  P Patriarca; R Cramer; S Moncalvo; F Rossi; D Romeo
Journal:  Arch Biochem Biophys       Date:  1971-07       Impact factor: 4.013

Review 3.  Mechanism of phagocytosis-associated oxidative metabolism in polymorphonuclear leucocytes and macrophages.

Authors:  F Rossi; D Romeo; P Patriarca
Journal:  J Reticuloendothel Soc       Date:  1972-08

4.  Involvement of calmodulin in phagocytotic respiratory burst of leukocytes.

Authors:  K Takeshige; S Minakami
Journal:  Biochem Biophys Res Commun       Date:  1981-03-31       Impact factor: 3.575

5.  Kinetic studies on the H2O2(O2-)-forming enzyme in guinea pig leukocytes.

Authors:  K Kakinuma; M Kaneda
Journal:  FEBS Lett       Date:  1980-02-25       Impact factor: 4.124

6.  Novel cytochrome b system in phagocytic vacuoles of human granulocytes.

Authors:  A W Segal; O T Jones
Journal:  Nature       Date:  1978-11-30       Impact factor: 49.962

7.  Studies on the metabolism of the renal glomerular basement membrane. Turnover measurements in the rat with the use of radiolabeled amino acids.

Authors:  R G Price; R G Spiro
Journal:  J Biol Chem       Date:  1977-12-10       Impact factor: 5.157

8.  Plasma membrane and phagosome localisation of the activated NADPH oxidase in elicited peritoneal macrophages of the guinea-pig.

Authors:  G Berton; P Bellavite; G de Nicola; P Dri; F Rossi
Journal:  J Pathol       Date:  1982-03       Impact factor: 7.996

9.  Comparison of NADH and NADPH oxidase activities in granules isolated from human polymorphonuclear leukocytes with a fluorometric assay.

Authors:  D Iverson; L R DeChatelet; J K Spitznagel; P Wang
Journal:  J Clin Invest       Date:  1977-02       Impact factor: 14.808

10.  The O2--producing enzyme of human neutrophils. Further properties.

Authors:  B M Babior; W A Peters
Journal:  J Biol Chem       Date:  1981-03-10       Impact factor: 5.157
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  6 in total

1.  Respiratory burst oxidase from human neutrophils: purification and some properties.

Authors:  M Markert; G A Glass; B M Babior
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

2.  Composition of partially purified NADPH oxidase from pig neutrophils.

Authors:  P Bellavite; O T Jones; A R Cross; E Papini; F Rossi
Journal:  Biochem J       Date:  1984-11-01       Impact factor: 3.857

3.  Phorbol 12-myristate 13-acetate activates an electrogenic H(+)-conducting pathway in the membrane of neutrophils.

Authors:  A Kapus; K Szászi; E Ligeti
Journal:  Biochem J       Date:  1992-02-01       Impact factor: 3.857

4.  Inhibition by gomisin C (a lignan from Schizandra chinensis) of the respiratory burst of rat neutrophils.

Authors:  J P Wang; S L Raung; M F Hsu; C C Chen
Journal:  Br J Pharmacol       Date:  1994-11       Impact factor: 8.739

5.  Macrophage deactivation. Altered kinetic properties of superoxide-producing enzyme after exposure to tumor cell-conditioned medium.

Authors:  S Tsunawaki; C F Nathan
Journal:  J Exp Med       Date:  1986-10-01       Impact factor: 14.307

6.  Production of oxygen-centered radicals by neutrophils and macrophages as studied by electron spin resonance (ESR).

Authors:  J V Bannister; W H Bannister
Journal:  Environ Health Perspect       Date:  1985-12       Impact factor: 9.031
  6 in total

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