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Literature DB >> 6862514

Technique for cloning bovine aortic endothelial cells.

Abstract

A simple reproducible method is described for isolating pure clonal populations of bovine aortic endothelial cells in culture. Single cells exposed to conditioned medium grow rapidly (clonal cell doubling time approximately 20 h) until a confluent monolayer is reached. This method provides virtually 100% cloning efficiency. In addition, the cell population doubling level of endothelial cell cultures, when continuously exposed to conditioned medium, is increased 40% over controls in fresh medium.

Entities: Species

Mesh：

Substances：
Culture Media

Year: 1983 PMID： 6862514 DOI： 10.1007/bf02619556

Source DB: PubMed Journal: In Vitro ISSN： 0073-5655

26 in total

1. Human vascular smooth muscle in culture. Growth and ultrastructure.

Authors: M A Gimbrone; R S Cotran
Journal: Lab Invest Date: 1975-07 Impact factor: 5.662

Review 2. The vascular endothelium-pathobiologic significance.

Authors: G Thorgeirsson; A L Robertson
Journal: Am J Pathol Date: 1978-12 Impact factor: 4.307

Review 3. Cellular aging--clonal senescence. A review (Part I).

Authors: G M Martin
Journal: Am J Pathol Date: 1977-11 Impact factor: 4.307

4. Cell size in aging monolayer cultures.

Authors: S B Greenberg; G L Grove; V J Cristofalo
Journal: In Vitro Date: 1977-05

5. Selection and characterization of bovine aortic endothelial cells.

Authors: S M Schwartz
Journal: In Vitro Date: 1978-12

6. Conditioned medium from endothelial cell cultures can restore the normal phenotypic expression of vascular endothelium maintained in vitro in the absence of fibroblast growth factor.

Authors: G Greenburg; I Vlodavsky; J M Foidart; D Gospodarowicz
Journal: J Cell Physiol Date: 1980-05 Impact factor: 6.384

7. Ability of endothelial cells to condition culture medium.

Authors: C M Gajdusek; S M Schwartz
Journal: J Cell Physiol Date: 1982-01 Impact factor: 6.384

8. Methods for microcarrier culture of bovine pulmonary artery endothelial cells avoiding the use of enzymes.

Authors: U S Ryan; M Mortara; C Whitaker
Journal: Tissue Cell Date: 1980 Impact factor: 2.466

9. Factors controlling the proliferative rate, final cell density, and life span of bovine vascular smooth muscle cells in culture.

Authors: D Gospodarowicz; K Hirabayashi; L Giguère; J P Tauber
Journal: J Cell Biol Date: 1981-06 Impact factor: 10.539

10. Human vascular endothelial cells in culture. Growth and DNA synthesis.

Authors: M A Gimbrone; R S Cotran; J Folkman
Journal: J Cell Biol Date: 1974-03 Impact factor: 10.539

17 in total

1. Tissue culture of human and canine thoracic duct endothelium.

Authors: D R Gnepp; W Chandler
Journal: In Vitro Cell Dev Biol Date: 1985-04

2. Specific antigen and organelle expression of a long-term rhesus endothelial cell line.

Authors: D A Lou; F N Hu
Journal: In Vitro Cell Dev Biol Date: 1987-02

3. Long-term culture of human corticotropin-secreting adenomas on extracellular matrix and evaluation of serum-free conditions. Secretory aspects.

Authors: M Westphal; P Jaquet; C B Wilson
Journal: Acta Neuropathol Date: 1986 Impact factor: 17.088

4. Carboxyl terminus of apolipoprotein A-I (ApoA-I) is necessary for the transport of lipid-free ApoA-I but not prelipidated ApoA-I particles through aortic endothelial cells.

Authors: Pascale M Ohnsorg; Lucia Rohrer; Damir Perisa; Andreas Kateifides; Angeliki Chroni; Dimitris Kardassis; Vassilis I Zannis; Arnold von Eckardstein
Journal: J Biol Chem Date: 2011-01-05 Impact factor: 5.157

10. Microtubule disruption stimulates DNA synthesis in bovine endothelial cells and potentiates cellular response to basic fibroblast growth factor.

Authors: L Liaw; S M Schwartz
Journal: Am J Pathol Date: 1993-09 Impact factor: 4.307