| Literature DB >> 6859525 |
C R Newton, A R Greene, G R Heathcliffe, T C Atkinson, D Holland, A F Markham, M D Edge.
Abstract
The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compared to those achieved with the same mixtures in other eluting solvents. The use of formamide systems is recommended for oligodeoxyribonucleotide purification in general and is particularly valuable where the oligonucleotide of interest is highly self-complementary and/or rich in deoxyguanosine residues.Entities:
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Year: 1983 PMID： 6859525 DOI： 10.1016/0003-2697(83)90046-5
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365