Literature DB >> 6857753

Synthesis of 2'(3')-O-DL-alanyl hexainosinic acid using T4 RNA ligase: suppression of the enzymic reverse transfer reaction by alkaline phosphatase.

A T Profy, K M Lo, D A Usher.   

Abstract

2'(3')-O-DL-Alanyl (Ip)5I was synthesized by a new method. An alanine ortho ester of inosine 5'-phosphate was added to (Ip)4I using the ATP-independent reaction of T4 RNA ligase, and the product was converted smoothly to the desired ester. The enzymic reverse transfer reaction was conveniently suppressed by the dephosphorylation of the adenosine 5'-phosphate coproduct, catalyzed in situ by alkaline phosphatase.

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Year:  1983        PMID： 6857753      PMCID： PMC325820          DOI： 10.1093/nar/11.5.1617

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  30 in total

1.  [Principal possibility of non-enzymatic aminoacylation of transport ribonucleic acids].

Authors:  B P Gottikh; A A Kraevskiĭ; T L Tsilevich; M K Kukhanova; A D Treboganov
Journal:  Dokl Akad Nauk SSSR       Date:  1974-11-01

2.  "Nonisomerizable" 2'-and 3'-O-aminoacyl dinucleoside phosphates. Chemical synthesis and acceptor activity in the ribosomal peptidyltransferase reaction.

Authors:  S Chládek; D Ringer; K Quiggle
Journal:  Biochemistry       Date:  1974-06-18       Impact factor: 3.162

3.  Conformation of oligoinosinates: chain-length dependence and comparison to other oligonucleotides.

Authors:  S Tazawa; I Tazawa; J L Alderfer; P O Ts'o
Journal:  Biochemistry       Date:  1972-09-12       Impact factor: 3.162

4.  Studies on acid hydrolases. IV. Isolation and characterization of spleen exonuclease.

Authors:  A Bernardi; G Bernardi
Journal:  Biochim Biophys Acta       Date:  1968-02-26

5.  Aminoacyl derivatives of nucleosides, nucleotides, and polynucleotides. 18. Synthesis of 2'(3')-O-aminoacyl derivatives of dinucleoside phosphates.

Authors:  S Chládek; J Zemlicka
Journal:  J Org Chem       Date:  1974-07-26       Impact factor: 4.354

6.  Peptidyl-donor substrates for ribosomal peptidyl transferase. Chemical synthesis and biological activity of N-acetyl aminoacyl di- and tri-nucleotides.

Authors:  J F Mercer; R H Symons
Journal:  Eur J Biochem       Date:  1972-06-23

7.  Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.

Authors:  R L Pearson; J F Weiss; A D Kelmers
Journal:  Biochim Biophys Acta       Date:  1971-02-11

8.  Synthesis of 2'(3')-O-glycyl derivatives of cytidylyl-(3'-5')-inosine and 2'-deoxycytidylyl-(3'-5')-adenosine.

Authors:  J Zemlicka; S Chladek
Journal:  Biochemistry       Date:  1971-04-27       Impact factor: 3.162

9.  The general synthetic route to amino acid esters of nucleotides and nucleoside-5'-triphosphates and some properties of these compounds.

Authors:  B P Gottikh; A A Krayevsky; N B Tarussova; P P Purygin; T L Tsilevich
Journal:  Tetrahedron       Date:  1970-09       Impact factor: 2.457

10.  Covalent joining of phenylalanine transfer ribonucleic acid half-molecules by T4 RNA ligase.

Authors:  G Kaufmann; U Z Littauer
Journal:  Proc Natl Acad Sci U S A       Date:  1974-09       Impact factor: 11.205
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  2 in total

1.  Biotin and fluorescent labeling of RNA using T4 RNA ligase.

Authors:  R W Richardson; R I Gumport
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

2.  Stereoselective aminoacylation of a dinucleoside monophosphate by the imidazolides of DL-alanine and N-(tert-butoxycarbonyl)-DL-alanine.

Authors:  A T Profy; D A Usher
Journal:  J Mol Evol       Date:  1984       Impact factor: 2.395
  2 in total

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