Literature DB >> 6857265

Transmission of integrated sea urchin histone genes by nuclear transplantation in Xenopus laevis.

L D Etkin, M Roberts.   

Abstract

Sea urchin histone genes contained in a recombinant plasmid pSp102 were microinjected into the cytoplasm of fertilized eggs of Xenopus laevis. By the late blastula stage, plasmid DNA sequences were detected comigrating with the high molecular weight cellular DNA (greater than 48 kilobases). Analysis of the DNA from injected embryos digested with various restriction endonuclease demonstrated that the injected DNA was integrated into the frog genome. Clones of embryos containing the pSp102 DNA sequences were produced by means of nuclear transplantation. Individuals of the same clone contain the pSp102 sequences integrated into similar chromosomal locations. These sites vary between different clones.

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Year:  1983        PMID: 6857265     DOI: 10.1126/science.6857265

Source DB:  PubMed          Journal:  Science        ISSN: 0036-8075            Impact factor:   47.728


  7 in total

1.  Persistence of bovine papillomavirus (pBPV-1) as an extrachromosomal element in tissues of microinjected Xenopus laevis.

Authors:  L D Etkin
Journal:  Nucleic Acids Res       Date:  1991-01-11       Impact factor: 16.971

2.  Chromosomal transplantation. The nuclear transplantation of colchicine-treated cells.

Authors:  J F Moruzzi; M S Ellinger
Journal:  Chromosoma       Date:  1984       Impact factor: 4.316

Review 3.  Xenopus: An emerging model for studying congenital heart disease.

Authors:  Erin Kaltenbrun; Panna Tandon; Nirav M Amin; Lauren Waldron; Chris Showell; Frank L Conlon
Journal:  Birth Defects Res A Clin Mol Teratol       Date:  2011-04-28

4.  Differential expression of the Xenopus laevis tadpole and adult beta-globin genes when injected into fertilized Xenopus laevis eggs.

Authors:  M M Bendig; J G Williams
Journal:  Mol Cell Biol       Date:  1984-03       Impact factor: 4.272

Review 5.  Transgenesis procedures in Xenopus.

Authors:  Albert Chesneau; Laurent M Sachs; Norin Chai; Yonglong Chen; Louis Du Pasquier; Jana Loeber; Nicolas Pollet; Michael Reilly; Daniel L Weeks; Odile J Bronchain
Journal:  Biol Cell       Date:  2008-09       Impact factor: 4.458

6.  Extrachromosomal DNA transformation of Caenorhabditis elegans.

Authors:  D T Stinchcomb; J E Shaw; S H Carr; D Hirsh
Journal:  Mol Cell Biol       Date:  1985-12       Impact factor: 4.272

Review 7.  Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support.

Authors:  Marko Horb; Marcin Wlizla; Anita Abu-Daya; Sean McNamara; Dominika Gajdasik; Takeshi Igawa; Atsushi Suzuki; Hajime Ogino; Anna Noble; Jacques Robert; Christina James-Zorn; Matthew Guille
Journal:  Front Physiol       Date:  2019-04-25       Impact factor: 4.566

  7 in total

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