The effects of chronic cobalt exposure on behavior and metallothionein levels in the adult rat.

Adult rats fed daily rations of laboratory chow laced with cobalt chloride were tested for operant (schedule-controlled) responding and conditioned suppression (CER). Animals exposed to 20 mg/kg Co, but not animals exposed to 5 mg/kg Co, lever pressed at a significantly slower rate than nontreated control subjects over the last 8 sessions of a 35 session operant training phase (1 session/day). Control and treatment subjects did not show significantly different CER performances. Nor were significant differences found on a test of mixed VI-2, DRO responding where subjects were required to lever press for reward during the first 15 min component and not lever press during the last 15 min component of the schedule. Testicular atrophy was evident among subjects exposed to 20 mg/kg Co. Atomic absorption spectrophotometric analyses revealed a dose-response effect regarding tissue accumulations of Co in blood, bone, brain, hair, small intestine, kidney, liver, and testes. Contrary to the biochemical pattern shown by some other heavy metals, prolonged Co exposure did not induce significant increases in metallothionein concentrations in the liver, kidney, or small intestine.