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Literature DB >> 6855753

Enzyme-linked immunosorbent assay for use with nuclear protein-DNA complex antigens.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for identification and quantification of nuclear antigens (nonhistone protein-DNA complexes from chromatin). Until now, the complement fixation assay has been the only immunoassay routinely applied to nonhistone protein-DNA complexes. The ELISA is considerably more sensitive than the micro-complement fixation test for assaying the immunospecificity of nuclear protein-DNA complexes. Dilutions of rabbit antisera as great as 1:6400 could be used to detect nanogram quantities of antigen, chicken reticulocyte chromatin or dehistonized rat liver chromatin.

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Year: 1983 PMID： 6855753 DOI： 10.1007/bf00230399

Source DB: PubMed Journal: Mol Cell Biochem ISSN： 0300-8177 Impact factor: 3.396

25 in total

1. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition.

Authors: E WASSERMAN; L LEVINE
Journal: J Immunol Date: 1961-09 Impact factor: 5.422

2. Tissue differences in antigenic properties of non-histone protein-DNA complexes.

Authors: F Chytil; T C Spelsberg
Journal: Nat New Biol Date: 1971-10-13

3. Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin G and virus-specific antibody.

Authors: H P Madore; A Baumgarten
Journal: J Clin Microbiol Date: 1979-10 Impact factor: 5.948

4. Antibodies against chromosomal HMG proteins stain the cytoplasm of mammalian cells.

Authors: M Bustin; N K Neihart
Journal: Cell Date: 1979-01 Impact factor: 41.582

5. Chromatin of the developing chick oviduct: changes in the acidic proteins.

Authors: T C Spelsberg; W M Mitchell; F Chytil; E M Wilson; B W O'Malley
Journal: Biochim Biophys Acta Date: 1973-07-27

6. Purification and partial characterization of nucleolar antigen-1 of the Novikoff hepatoma.

Authors: F Marashi; F M Davis; R K Busch; H E Savage; H Busch
Journal: Cancer Res Date: 1979-01 Impact factor: 12.701

7. Distribution patterns of three subfractions of drosophila nonhistone chromosomal proteins: possible correlations with gene activity.

Authors: L M Silver; S C Elgin
Journal: Cell Date: 1977-08 Impact factor: 41.582

Enzyme-linked immunosorbent assay for use with nuclear protein-DNA complex antigens.

1. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition.

2. Tissue differences in antigenic properties of non-histone protein-DNA complexes.

3. Enzyme-linked protein A: an enzyme-linked immunosorbent assay reagent for detection of human immunoglobulin G and virus-specific antibody.

4. Antibodies against chromosomal HMG proteins stain the cytoplasm of mammalian cells.

5. Chromatin of the developing chick oviduct: changes in the acidic proteins.

6. Purification and partial characterization of nucleolar antigen-1 of the Novikoff hepatoma.

7. Distribution patterns of three subfractions of drosophila nonhistone chromosomal proteins: possible correlations with gene activity.

8. Immunochemical analysis of nonhistone proteins.

9. Nucleolar antigen found in several human tumors but not in the nontumor tissues studied.

10. Colon tumor specific nuclear antigen with potential as a pre-tumor diagnostic probe.

1. Hepatoma-associated nonhistone chromosomal proteins are present in active chromatin.