Literature DB >> 6853735

A fluorimetric method for the measurement of pyridoxal and pyridoxal phosphate in human plasma and leucocytes, and its application to patients with sideroblastic marrows.

G P Smith, D Samson, T J Peters.   

Abstract

A highly sensitive fluorimetric assay for the measurement of pyridoxal and pyridoxal phosphate in biological tissues is described. The method involves the enzymic hydrolysis of pyridoxal phosphate to pyridoxal. The pyridoxal (free or total) is separated on an anion-exchange column, concentrated by cation-exchange chromatography and reacted with potassium cyanide under slightly alkaline conditions to form 4-pyridoxolactone, a highly fluorescent compound. The method is applied to the measurement of pyridoxal, pyridoxal phosphate and total pyridoxal in plasma and neutrophils from control subjects and patients with sideroblastic marrow and identified the patient with pyridoxine-responsive sideroblastic anaemia.

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Year:  1983        PMID： 6853735      PMCID： PMC498354          DOI： 10.1136/jcp.36.6.701

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  19 in total

1.  Pyridoxal phosphate breakdown by an alkaline-phosphatase preparation.

Authors:  J M TURNER
Journal:  Biochem J       Date:  1961-09       Impact factor: 3.857

2.  Environmental Health in Community Growth: An Administrative Guide to Metropolitan Area Sanitation Practices Committee, Engineering and Sanitarian Section, APHA.

Authors:  F B Taylor; D B Lee; W Vanheuvelen; I Grossman; H H Hasson; H C Mitchell; D R Olive; E H Pearl; J W Price; I F Shull; W F Smith; J A Salvato
Journal:  Am J Public Health Nations Health       Date:  1963-05

3.  Arteriosclerotic lesions in pyridoxine-deficient monkeys.

Authors:  J F RINEHART; L D GREENBERG
Journal:  Am J Pathol       Date:  1949-05       Impact factor: 4.307

4.  Effect of pyridoxine plus chronic phlebotomy on the function and morphology of bone marrow and liver in pyridoxine-responsive sideroblastic anemia.

Authors:  J D Hines
Journal:  Semin Hematol       Date:  1976-04       Impact factor: 3.851

5.  Plasma binding of vitamin B6 compounds.

Authors:  B B Anderson; P A Newmark; M Rawlins; R Green
Journal:  Nature       Date:  1974-08-09       Impact factor: 49.962

6.  Enzymatic microassay of pyridoxal-5'-phosphate using L-tyrosine apodecarboxylase and L-(1-14C) tyrosine.

Authors:  H N Bhagavan; J M Koogler; D B Coursin
Journal:  Int J Vitam Nutr Res       Date:  1976       Impact factor: 1.784

7.  Evidence for the regulation of pyridoxal 5-phosphate formation in liver by pyridoxamine (pyridoxine) 5 -phosphate oxidase.

Authors:  A H Merrill; K Horiike; D B McCormick
Journal:  Biochem Biophys Res Commun       Date:  1978-08-14       Impact factor: 3.575

8.  Effect of oral contraceptives on the plasma concentration of pyridoxal phosphate.

Authors:  L Lumeng; R E Cleary; T K Li
Journal:  Am J Clin Nutr       Date:  1974-04       Impact factor: 7.045

9.  Some effects of chronic ethanol feeding on vitamin B 6 deficiency in the rat.

Authors:  S W French; J Castagna
Journal:  Lab Invest       Date:  1967-04       Impact factor: 5.662

10.  Subcellular localization and properties of pyridoxal phosphate phosphatases of human polymorphonuclear leukocytes and their relationship to acid and alkaline phosphatase.

Authors:  G P Smith; T J Peters
Journal:  Biochim Biophys Acta       Date:  1981-10-13
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  2 in total

1.  Vitamin B6 concentrations in patients with chronic liver disease and hepatocellular carcinoma.

Authors:  D I Thurnham; R Singkamani; R D Situnayake; M Davis
Journal:  Br Med J (Clin Res Ed)       Date:  1986-09-13

2.  Vitamin B6 concentrations in patients with chronic liver disease and hepatocellular carcinoma.

Authors:  S N Zaman; J M Tredger; P J Johnson; R Williams
Journal:  Br Med J (Clin Res Ed)       Date:  1986-07-19
  2 in total

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