Literature DB >> 6853547

The use of monoclonal antibodies to fragments of chicken type IV collagen in structural and localization studies.

R Mayne, R D Sanderson, H Wiedemann, J M Fitch, T F Linsenmayer.   

Abstract

In previous experiments, three pepsin-resistant fragments of type IV collagen were isolated from chicken gizzards and designated 7S, F3, and (F1)2F2 (Mayne, R., and Zettergren, J. G. (1980) Biochemistry 19, 4065-4072). In the present experiments, a series of monoclonal antibodies to type IV collagen were prepared, each one of which recognized an epitope present in only one of the three fragments. A high molecular weight fraction of type IV collagen (designated 7S + arms (215 nm)) was isolated after agarose gel filtration and characterized by electron microscopy after rotary shadowing and by gel electrophoresis. Analysis of 7S + arms (215 nm) by inhibition enzyme-linked immunosorbent assay demonstrated the presence of the epitopes for 7S and F3 but not for (F1)2F2. This result, therefore, provides additional evidence that the order of the pepsin-resistant fragments of chicken type IV collagen is 7S-F3-(F1)2F2.

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Year:  1983        PMID： 6853547

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  9 in total

1.  Characterization of monoclonal antibody specific for human type II collagen: possible implication in collagen-induced arthritis.

Authors:  T Hirofuji; K Kakimoto; H Hori; Y Nagai; K Saisho; A Sumiyoshi; T Koga
Journal:  Clin Exp Immunol       Date:  1985-10       Impact factor: 4.330

2.  Monoclonal antibodies to human type IV collagen: useful reagents to demonstrate the heterotrimeric nature of the molecule.

Authors:  B F Odermatt; A B Lang; J R Rüttner; K H Winterhalter; B Trüeb
Journal:  Proc Natl Acad Sci U S A       Date:  1984-12       Impact factor: 11.205

3.  The spatial organization of Descemet's membrane-associated type IV collagen in the avian cornea.

Authors:  J M Fitch; D E Birk; C Linsenmayer; T F Linsenmayer
Journal:  J Cell Biol       Date:  1990-04       Impact factor: 10.539

4.  Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.

Authors:  Z Yablonka-Reuveni; B Christ; J M Benson
Journal:  Anat Embryol (Berl)       Date:  1998-06

5.  Location of the integrin complex and extracellular matrix molecules at the chicken myotendinous junction.

Authors:  S Swasdison; R Mayne
Journal:  Cell Tissue Res       Date:  1989-09       Impact factor: 5.249

6.  Monoclonal antibodies against chicken type IV and V collagens: electron microscopic mapping of the epitopes after rotary shadowing.

Authors:  R Mayne; H Wiedemann; M H Irwin; R D Sanderson; J M Fitch; T F Linsenmayer; K Kühn
Journal:  J Cell Biol       Date:  1984-05       Impact factor: 10.539

7.  Collagen type I and type V are present in the same fibril in the avian corneal stroma.

Authors:  D E Birk; J M Fitch; J P Babiarz; T F Linsenmayer
Journal:  J Cell Biol       Date:  1988-03       Impact factor: 10.539

8.  Thermal stability of the helical structure of type IV collagen within basement membranes in situ: determination with a conformation-dependent monoclonal antibody.

Authors:  T F Linsenmayer; E Gibney; J M Fitch; J Gross; R Mayne
Journal:  J Cell Biol       Date:  1984-10       Impact factor: 10.539

9.  Developmental acquisition of basement membrane heterogeneity: type IV collagen in the avian lens capsule.

Authors:  J M Fitch; R Mayne; T F Linsenmayer
Journal:  J Cell Biol       Date:  1983-09       Impact factor: 10.539
  9 in total

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