Literature DB >> 6847612

A simple assay to study protein-mediated lipid exchange by fluorescence polarization.

Y H Xü, K Gietzen, H J Galla, E Sackmann.   

Abstract

We investigated the protein-mediated phospholipid transfer between small vesicles by fluorescence polarization measurements with diphenylhexatriene as optical probe. Thermotropic-phase-transition curves were taken after mixing two vesicle preparations of lipids exhibiting different gel-to-liquid phase transitions. From the heights of each phase-transition step we were able to follow the lipid transfer process without separating the two vesicle preparations.

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Year:  1983        PMID： 6847612      PMCID： PMC1154080          DOI： 10.1042/bj2090257

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Purification of phospholipid exchange proteins from beef heart.

Authors:  D B Zilversmit; L W Johnson
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600

Review 2.  Transfer of phospholipids between membranes.

Authors:  K W Wirtz
Journal:  Biochim Biophys Acta       Date:  1974-09-16

3.  The asymmetric distribution of phospholipids in the human red cell membrane. A combined study using phospholipases and freeze-etch electron microscopy.

Authors:  A J Verkleij; R F Zwaal; B Roelofsen; P Comfurius; D Kastelijn; L L van Deenen
Journal:  Biochim Biophys Acta       Date:  1973-10-11

4.  Transfer of phosphatidylcholine between liposomes.

Authors:  J A Hellings; H H Kamp; K W Wirtz; L L Van Deenen
Journal:  Eur J Biochem       Date:  1974-09-16

5.  Phosphatidylcholine exchange protein from beef liver.

Authors:  H H Kamp; K W Wirtz
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  Phosphatidyl-ethanolamine: differential labelling in intact cells and cell ghosts of human erythrocytes by a membrane-impermeable reagent.

Authors:  M S Bretscher
Journal:  J Mol Biol       Date:  1972-11-28       Impact factor: 5.469

7.  Participation of soluble liver proteins in the exchange of membrane phospholipids.

Authors:  K W Wirtz; D B Zilversmit
Journal:  Biochim Biophys Acta       Date:  1969-10-14

8.  The protein-mediated transfer of phosphatidylcholine between membranes. The effect of membrane lipid composition and ionic composition of the medium.

Authors:  K W Wirtz; W S Van Kessel; H H Kamp; R A Demel
Journal:  Eur J Biochem       Date:  1976-01-15

9.  Protein-catalyzed phospholipid exchange between gel and liquid-crystalline phospholipid vesicles.

Authors:  A M Kasper; G M Helmkamp
Journal:  Biochemistry       Date:  1981-01-06       Impact factor: 3.162

Review 10.  Membrane asymmetry.

Authors:  J E Rothman; J Lenard
Journal:  Science       Date:  1977-02-25       Impact factor: 47.728
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  1 in total

1.  Protein-mediated lipid transfer. The effects of lipid-phase transition and of charged lipids.

Authors:  Y H Xü; K Gietzen; H J Galla; E Sackmann
Journal:  Biochem J       Date:  1983-07-01       Impact factor: 3.857
  1 in total

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