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Literature DB >> 6846845

A high recovery method for concentrating microgram quantities of protein from large volumes of solution.

D Mahuran, P Clements, M Carrella, P M Strasberg.

Abstract

A method is presented for the recovery of 40-80% of the protein from a 1 microgram/ml solution. The final protein pellet is free of detergent and other ionic compounds and is thus compatible with any denaturing solution. The primary structure of the protein is unaffected by the procedure, making the final pellet an ideal sample for any analytical procedure to determine protein structure.

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Year: 1983 PMID： 6846845 DOI： 10.1016/0003-2697(83)90585-7

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

11 in total

1. Human N-acetylgalactosamine-4-sulphatase biosynthesis and maturation in normal, Maroteaux-Lamy and multiple-sulphatase-deficient fibroblasts.

Authors: J A Taylor; G J Gibson; D A Brooks; J J Hopwood
Journal: Biochem J Date: 1990-06-01 Impact factor: 3.857

9. Human N-acetylgalactosamine-4-sulphate sulphatase. Purification, monoclonal antibody production and native and subunit Mr values.

Authors: G J Gibson; G T Saccone; D A Brooks; P R Clements; J J Hopwood
Journal: Biochem J Date: 1987-12-15 Impact factor: 3.857

10. Human liver sulphamate sulphohydrolase. Determinations of native protein and subunit Mr values and influence of substrate agylcone structure on catalytic properties.

Authors: C Freeman; J J Hopwood
Journal: Biochem J Date: 1986-02-15 Impact factor: 3.857

A high recovery method for concentrating microgram quantities of protein from large volumes of solution.

1. Human N-acetylgalactosamine-4-sulphatase biosynthesis and maturation in normal, Maroteaux-Lamy and multiple-sulphatase-deficient fibroblasts.

2. Partial amino acid sequence of porcine 1,25-dihydroxyvitamin D3 receptor isolated by immunoaffinity chromatography.

3. Human liver iduronate-2-sulphatase. Purification, characterization and catalytic properties.

4. Immunopurification and characterization of human alpha-L-iduronidase with the use of monoclonal antibodies.

5. Differential targeting of closely related ECM glycoproteins: the pherophorin family from Volvox.

6. Human liver N-acetylglucosamine-6-sulphate sulphatase. Purification and characterization.

7. Human liver N-acetylgalactosamine 6-sulphatase. Purification and characterization.

8. Human liver glucuronate 2-sulphatase. Purification, characterization and catalytic properties.

9. Human N-acetylgalactosamine-4-sulphate sulphatase. Purification, monoclonal antibody production and native and subunit Mr values.

10. Human liver sulphamate sulphohydrolase. Determinations of native protein and subunit Mr values and influence of substrate agylcone structure on catalytic properties.