| Literature DB >> 6846823 |
K Imai, T Toyo'oka, Y Watanabe.
Abstract
Ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F) was newly synthesized as a fluorogenic reagent for thiol compounds. Its reactivity to glutathione is superior to its 7-chloro analog, SBD-Cl. The optimal reaction condition was selected at 60 degrees C and pH 9.5 for 1 h. The detection limits for glutathione, coenzyme A, cysteamine, homocysteine, N-acetylcysteine, cysteine, and D-3-mercaptomethylpropanoyl-L-proline (captopril) were 100, 120, 160, 330, 390, 3600, and 150 pmol/ml, respectively. Proline and alanine did not give fluorescence under this condition.Entities:
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Year: 1983 PMID: 6846823 DOI: 10.1016/0003-2697(83)90404-9
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365