Literature DB >> 6846803

A method for distinguishing Cu,Zn- and Mn-containing superoxide dismutases.

B L Geller, D R Winge.   

Abstract

A procedure has been developed to distinguish between the two forms of eukaryotic superoxide dismutases using a common activity assay. Treatment of cellular fractions with 2% sodium dodecyl sulfate at 37 degrees C for 30 min selectively inactivates the mitochondrial, manganese-containing variant without affecting the cytosolic copper, zinc-superoxide dismutase. After removing excess sodium dodecyl sulfate by precipitation with potassium chloride, the supernate is assayed using the xanthine oxidase-cytochrome c method.

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Year:  1983        PMID： 6846803     DOI： 10.1016/0003-2697(83)90348-2

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

1.  A spectrophotometric assay for superoxide dismutase activities in crude tissue fractions.

Authors:  H Kuthan; H J Haussmann; J Werringloer
Journal:  Biochem J       Date:  1986-07-01       Impact factor: 3.857

2.  Early changes in myocardial antioxidant enzymes in rats treated with adriamycin.

Authors:  Timao Li; Igor Danelisen; Pawan K Singal
Journal:  Mol Cell Biochem       Date:  2002-03       Impact factor: 3.396

3.  NADH Induces the Generation of Superoxide Radicals in Leaf Peroxisomes.

Authors:  L A Del Río; V M Fernández; F L Rupérez; L M Sandalio; J M Palma
Journal:  Plant Physiol       Date:  1989-03       Impact factor: 8.340

4.  Antioxidative enzymes in irradiated rat brain-indicators of different regional radiosensitivity.

Authors:  Ana Todorović; Snežana Pejić; Vesna Stojiljković; Ljubica Gavrilović; Nataša Popović; Ivan Pavlović; Zorica S Saičić; Snežana B Pajović
Journal:  Childs Nerv Syst       Date:  2015-07-05       Impact factor: 1.475

5.  Effects of estradiol benzoate and progesterone on superoxide dismutase activity in the rat liver.

Authors:  J Kasapović; S B Pajović; D T Kanazir; J V Martinović
Journal:  J Endocrinol Invest       Date:  1997-04       Impact factor: 4.256

6.  Properties of extracellular superoxide dismutase from human lung.

Authors:  S L Marklund
Journal:  Biochem J       Date:  1984-05-15       Impact factor: 3.857

7.  High-level expression of enzymatically active human Cu/Zn superoxide dismutase in Escherichia coli.

Authors:  J R Hartman; T Geller; Z Yavin; D Bartfeld; D Kanner; H Aviv; M Gorecki
Journal:  Proc Natl Acad Sci U S A       Date:  1986-10       Impact factor: 11.205

8.  Antioxidant effects of ethyl acetate extract of Desmodium gangeticum root on myocardial ischemia reperfusion injury in rat hearts.

Authors:  Gino A Kurian; Srilalitha Suryanarayanan; Archana Raman; Jose Padikkala
Journal:  Chin Med       Date:  2010-01-22       Impact factor: 5.455

9.  Endogenous ghrelin increases in adriamycin-induced heart failure rats.

Authors:  Z Xu; W Wu; X Zhang; G Liu
Journal:  J Endocrinol Invest       Date:  2007-02       Impact factor: 4.256

10.  Expression of antioxidant enzymes in rat kidney during ischemia-reperfusion injury.

Authors:  I Singh; S Gulati; J K Orak; A K Singh
Journal:  Mol Cell Biochem       Date:  1993-08-25       Impact factor: 3.396
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