Literature DB >> 6846796

Determination of malonaldehyde in biological materials by high-pressure liquid chromatography.

R P Bird, S S Hung, M Hadley, H H Draper.   

Abstract

An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a mu Bondapak C18 column, and measuring the absorbance using a 546-nm interference filter. The method was found to be specific for malonaldehyde in several food and feed samples. Under routine assay conditions, a coefficient of variability of 7.0% was obtained for samples containing 1-2 microgram of malonaldehyde per gram (instrument detection limit 1 ng). This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm.

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Year:  1983        PMID： 6846796     DOI： 10.1016/0003-2697(83)90371-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  21 in total

1.  Response of urinary malondialdehyde to factors that stimulate lipid peroxidation in vivo.

Authors:  S N Dhanakoti; H H Draper
Journal:  Lipids       Date:  1987-09       Impact factor: 1.880

2.  Gas chromatographic analysis of free and bound malonaldehyde in rat liver homogenates.

Authors:  T Ichinose; M G Miller; T Shibamoto
Journal:  Lipids       Date:  1989-10       Impact factor: 1.880

3.  Increased urinary excretion of 2-thiobarbituric acid reactants in rats exposed to diesel engine exhaust.

Authors:  H Seto; T Suzuki; T Ohkubo; T Kanoh
Journal:  Bull Environ Contam Toxicol       Date:  1990-10       Impact factor: 2.151

4.  Urinary response to in vivo lipid peroxidation induced by vitamin E deficiency.

Authors:  H S Lee; D W Shoeman; A S Csallany
Journal:  Lipids       Date:  1992-02       Impact factor: 1.880

Review 5.  Free radicals, reactive oxygen species and human disease: a critical evaluation with special reference to atherosclerosis.

Authors:  B Halliwell
Journal:  Br J Exp Pathol       Date:  1989-12

6.  Thiobarbituric acid-reactive substances from peroxidized lipids.

Authors:  H Kosugi; T Kojima; K Kikugawa
Journal:  Lipids       Date:  1989-10       Impact factor: 1.880

7.  Characteristics of the thiobarbituric acid reactivity of human urine as a possible consequence of lipid peroxidation.

Authors:  H Kosugi; T Kojima; K Kikugawa
Journal:  Lipids       Date:  1993-04       Impact factor: 1.880

8.  Iron catalyzed oxidation of trout diets and its effect on the growth and physiological response of rainbow trout.

Authors:  L M Desjardins; B D Hicks; J W Hilton
Journal:  Fish Physiol Biochem       Date:  1987-06       Impact factor: 2.794

9.  Simultaneous determination of the main molecular species of soybean phosphatidylcholine or phosphatidylethanolamine and their corresponding hydroperoxides obtained by lipoxygenase treatment.

Authors:  P Therond; M Couturier; J F Demelier; F Lemonnier
Journal:  Lipids       Date:  1993-03       Impact factor: 1.880

10.  [Ischemia/reperfusion damage of the liver caused by free radicals--direct radical detection using electron spin resonance (ESR)].

Authors:  R Kunz; H A Brune; U Ziegler; M Marzinzig; H G Beger
Journal:  Langenbecks Arch Chir       Date:  1991
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