| Literature DB >> 6846791 |
Abstract
Chymotrypsin can be determined with an NADH-coupled assay. Hydrolysis of the substrate benzoyltyrosine ethyl ester is monitored by coupling the liberation of ethanol to the production of NADH and determining the NADH spectrophotometrically or fluorometrically. Nanogram quantities of chymotrypsin can be determined in milliliter volumes. With these microfluorescence methods this assay can be performed in a final volume of less than a nanoliter, allowing determination of femtogram quantities of chymotrypsin, the amount present in an individual zymogen granule.Entities:
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Year: 1983 PMID： 6846791 DOI： 10.1016/0003-2697(83)90360-3
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365