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Literature DB >> 6841142

Enhanced membrane fluorescence of CDC-labelled paramecium subsequent to removal of surface components.

Abstract

Cytofluorimetric analysis of cycloheptaamylose-dansyl chloride (CDC) labelled Paramecium indicates that after mild trypsin removal of surface components the localization of CDC on the outer surface of living cells was not modified by the treatment. After such treatment the intensity of fluorescence emission was found about 3-fold higher in treated single cell than in the untreated one. These findings indicate that CDC labelling can be used to follow alteration occurred on the membrane of the living cell prior to labelling.

Entities: Chemical

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Year: 1983 PMID： 6841142 DOI： 10.1007/bf00506559

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

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References

11 in total

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Authors: E STEERS
Journal: Proc Natl Acad Sci U S A Date: 1962-05-15 Impact factor: 11.205

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Authors: G L Nicolson
Journal: Biochim Biophys Acta Date: 1976-04-13

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Authors: S P Hawkes; J C Bartholomew
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

7. Enhancement of the fluorescence intensity of dansyl amino acids in aqueous media and its application to assay of amino acids.

Authors: T Kinoshita; F Iinuma; A Tsuji
Journal: Biochem Biophys Res Commun Date: 1973-04-02 Impact factor: 3.575

Enhanced membrane fluorescence of CDC-labelled paramecium subsequent to removal of surface components.

1. A comparison of the tryptic peptides obtained from immobilization antigens of Paramecium aurelia.

2. Transmembrane control of the receptors on normal and tumor cells. I. Cytoplasmic influence over surface components.

3. Quantitative determination of transformed cells in a mixed population by stimultaneous fluorescence analysis of cell surface and DNA an individual cells.

4. The use of fluorescamine as a probe for labeling the outer surface of the plasma membrane.

5. Studies on the surface coat of Paramecium aurelia. II. Relationship to the immobilization antigen.

6. Organization of glycolipids and glycoproteins in surface membranes: dependency on cell cycle and on transformation.

7. Enhancement of the fluorescence intensity of dansyl amino acids in aqueous media and its application to assay of amino acids.

8. Concerning the tertiary structure of the soluble surface proteins of Paramecium.

9. Cell multiplication following partial enzymatic removal of surface coat.

10. Cycloheptaamylose-dansyl chloride complex as a fluorescent label of surface membranes in living ciliates.