Is erythrocyte membrane phospholipid organisation abnormal in Duchenne muscular dystrophy?

Many of the abnormalities reported in erythrocytes from Duchenne muscular dystrophy (DMD) patients, including alterations in the physical state of the membrane as determined by electron spin resonance, could arise from changes in the lipid components. Although several investigators have shown there to be no compositional differences between normal and DMD erythrocytes, there still exists the hitherto unexplored possibility that the normal asymmetric organisation of the lipids may be deranged in the disease. This report is concerned with the probing of the asymmetric transbilayer distribution of the glycerophospholipids using phospholipase A2 from bee venom. Our results suggest that more phosphatidyl choline (PC) is available for attack by the enzyme in the outer leaflet of the bilayer in DMD erythrocytes than in normals. This may be due to a greater than normal proportion of the PC being present in the outer leaflet or to enhanced transbilayer movement of PC molecules from the inner leaflet. Whichever the explanation, these findings indicate an organisational abnormality in DMD erythrocyte membranes.