Literature DB >> 6838822

Purification and characterization of a cysteine dioxygenase from the yeast phase of Histoplasma capsulatum.

V Kumar, B Maresca, M Sacco, R Goewert, G S Kobayashi, G Medoff.   

Abstract

A cysteine dioxygenase, cysteine oxidase (EC 1.13.11.20), has been purified from the cytosolic fraction of yeast phase cells of the dimorphic fungus Histoplasma capsulatum. The cysteine oxidase is an iron-containing dioxygenase with a molecular weight of 10500 (+/- 1500) and is present only in the yeast phase of the fungus. The enzyme is highly specific for L-cysteine, with a Km of 2 X 10(-5) M in vitro. The product of cysteine oxidation is cysteinesulfinic acid, as analyzed by thin-layer chromatography and mass spectroscopy. To our knowledge, this is the first cysteine oxidase isolated from a fungus, and it probably plays an important role in the mycelial to yeast phase transition of H. capsulatum during which redox potential and cysteine levels are crucial factors.

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Year:  1983        PMID： 6838822     DOI： 10.1021/bi00273a009

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  9 in total

1.  Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation.

Authors:  A M Gehring; J R Nodwell; S M Beverley; R Losick
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2.  Identification and characterization of bacterial cysteine dioxygenases: a new route of cysteine degradation for eubacteria.

Authors:  John E Dominy; Chad R Simmons; P Andrew Karplus; Amy M Gehring; Martha H Stipanuk
Journal:  J Bacteriol       Date:  2006-08       Impact factor: 3.490

3.  History of medical mycology in the united states.

Authors:  A Espinel-Ingroff
Journal:  Clin Microbiol Rev       Date:  1996-04       Impact factor: 26.132

4.  Correlation between pathogenicity and temperature sensitivity in different strains of Histoplasma capsulatum.

Authors:  G Medoff; B Maresca; A M Lambowitz; G Kobayashi; A Painter; M Sacco; L Carratu
Journal:  J Clin Invest       Date:  1986-12       Impact factor: 14.808

5.  Cystine catabolism in mycelia of Microsporum gypseum, a dermatophytic fungus.

Authors:  J Kunert; H G Trüper
Journal:  Arch Microbiol       Date:  1986-07       Impact factor: 2.552

Review 6.  Dimorphism in Histoplasma capsulatum: a model for the study of cell differentiation in pathogenic fungi.

Authors:  B Maresca; G S Kobayashi
Journal:  Microbiol Rev       Date:  1989-06

Review 7.  Histoplasma variation and adaptive strategies for parasitism: new perspectives on histoplasmosis.

Authors:  L G Eissenberg; W E Goldman
Journal:  Clin Microbiol Rev       Date:  1991-10       Impact factor: 26.132

8.  Identifying phase-specific genes in the fungal pathogen Histoplasma capsulatum using a genomic shotgun microarray.

Authors:  Lena Hwang; Davina Hocking-Murray; Adam K Bahrami; Margareta Andersson; Jasper Rine; Anita Sil
Journal:  Mol Biol Cell       Date:  2003-03-20       Impact factor: 4.138

9.  Comparative Proteomic Analysis of Histoplasma capsulatum Yeast and Mycelium Reveals Differential Metabolic Shifts and Cell Wall Remodeling Processes in the Different Morphotypes.

Authors:  Marcos Abreu Almeida; Lilian Cristiane Baeza; Rodrigo Almeida-Paes; Alexandre Melo Bailão; Clayton Luiz Borges; Allan Jefferson Guimarães; Célia Maria Almeida Soares; Rosely Maria Zancopé-Oliveira
Journal:  Front Microbiol       Date:  2021-06-11       Impact factor: 5.640
  9 in total

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