Molecular weights of the major DNA polymerases in a higher plant, Pisum sativum L. (PEA).

The molecular weights of the soluble (alpha-like) and chromatin-bound (beta-like) DNA polymerases of pea have been determined. The bulk of the soluble activity consists of molecular species of ca 101,500 and ca 140,000 molecular weights. Smaller (49,000) and larger (182,000 and 234,000) species are also observed in some preparations. The chromatin-bound DNA polymerase exhibits a molecular weight of ca 50,000, although a larger (ca 88,000) species is also detected under conditions which favour aggregate formation.