Lymphocytic choriomeningitis virus. IV. Electron microscopic investigation of the virion.

The structure of lymphocytic choriomeningitis virus (LCM virus) was investigated by a variety of conventional as well as novel electron microscopic procedures. Thin sections of infected cells revealed the characteristic arenavirus entities whose interiors contain ribosome-like granules but look otherwise empty. In contrast, most thin-sectioned virus particles from infectious cell culture fluid, both untreated and highly purified with little loss of initial infectivity, appeared to be filled with rather homogeneous cores. Cores rather than granules were also found in positively contrasted whole and thin-sectioned virus particles. We favor the explanation that the sandy grains, which have given this group of viruses its name, are altered cores that happen to look like ribosomes. However, the alternative cannot yet be excluded, namely, that LCM virus-infected cells produce two types of particles, of which only the core-containing ones represent virions.