Literature DB >> 6834009

Affinity chromatography of human fibroblast interferon (IFN-beta 1) by monoclonal antibody columns.

D Novick, Z Eshhar, O Gigi, Z Marks, M Revel, M Rubinstein.   

Abstract

Eight hybridomas secreting monoclonal antibodies specific to human fibroblast interferon (IFN-beta 1) were identified by a new screening procedure based on immune precipitation with a second antibody and recovery of interferon activity from the immune precipitate. Immunoadsorbents were prepared from these monoclonal antibodies and used for purification of IFN-beta 1. Electrophoretically pure IFN-beta 1, having a specific activity of 3 X 10(8) to 6 X 10(8) units/mg was obtained by a single passage of partially purified IFN-beta 1 on these immunoadsorbents.

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Year:  1983        PMID： 6834009     DOI： 10.1099/0022-1317-64-4-905

Source DB:  PubMed          Journal:  J Gen Virol        ISSN： 0022-1317            Impact factor:   3.891


  7 in total

1.  Two antiviral proteins from tobacco: purification and characterization by monoclonal antibodies to human beta-interferon.

Authors:  O Edelbaum; N Ilan; G Grafi; N Sher; Y Stram; D Novick; N Tal; I Sela; M Rubinstein
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

2.  Localization of interferons and interleukin 2 in polymyositis and muscular dystrophy.

Authors:  D A Isenberg; D Rowe; M Shearer; D Novick; P C Beverley
Journal:  Clin Exp Immunol       Date:  1986-02       Impact factor: 4.330

3.  HLA class I and II, interferon, interleukin 2, and the interleukin 2 receptor expression on labial biopsy specimens from patients with Sjögren's syndrome.

Authors:  D Rowe; M Griffiths; J Stewart; D Novick; P C Beverley; D A Isenberg
Journal:  Ann Rheum Dis       Date:  1987-08       Impact factor: 19.103

4.  Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.

Authors:  G Merlin; J Chebath; P Benech; R Metz; M Revel
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

5.  Structure and expression of cDNA and genes for human interferon-beta-2, a distinct species inducible by growth-stimulatory cytokines.

Authors:  A Zilberstein; R Ruggieri; J H Korn; M Revel
Journal:  EMBO J       Date:  1986-10       Impact factor: 11.598

6.  Monoclonal antibodies to human interferon-gamma: production, affinity purification and radioimmunoassay.

Authors:  D Novick; Z Eshhar; D G Fischer; J Friedlander; M Rubinstein
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

7.  Autogenous production of interferon-beta switches on HLA genes during differentiation of histiocytic lymphoma U937 cells.

Authors:  A Yarden; H Shure-Gottlieb; J Chebath; M Revel; A Kimchi
Journal:  EMBO J       Date:  1984-05       Impact factor: 11.598
  7 in total

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