Dopamine uptake in striatal synaptosomes exposed to peroxidation "in vitro".

The uptake of [3H]dopamine by synaptosomes from rat brain striatum was biphasic, the rapid phase requiring less than 1 min for completion and the slower phase occurring over a few minutes. This uptake of labeled dopamine was enhanced by prior exposure of the synaptosomes to Fe2+, which gives rise to peroxidation of the synaptosomal lipids. Both lipid peroxidation and enhancement of dopamine uptake were unaffected by the presence of inhibitors of monoamine oxidase but were blocked by inclusion of omicron-phenanthroline in the preincubation medium. It is concluded that lipid peroxidation may be involved in certain neuropsychiatric disorders such as seizures evoked by exposure to high oxygen pressures.