Literature DB >> 6830220

Fluorogenic selective and differential medium for isolation of fecal streptococci.

K J Littel, P A Hartman.   

Abstract

Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, was hydrolyzed by Streptococcus bovis and S. faecium and its biotypes. 4-Methylumbelliferone-alpha-D-galactoside and a colorimetric starch substrate were incorporated into the fecal streptococcal selective medium of Donnelly and Hartman (Appl. Environ. Microbiol. 35:576-581, 1978). Three phenotypic groups were identifiable on the new fluorescent gentamicin-thallous-carbonate agar: (i) starch hydrolysis and fluorescence (S. bovis), (ii) no starch hydrolysis but fluorescence (S. faecium and its biotypes), and (iii) no starch hydrolysis or fluorescence (S. faecalis, S. avium, S. equinus, S. mitis, and S. salivarius). Of the presumptive identifications from sewage, swine, and bovine samples, 86% were confirmed as being correct. The new medium has potential application in water, food, environmental, and possibly clinical microbiology.

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Year:  1983        PMID: 6830220      PMCID: PMC242334          DOI: 10.1128/aem.45.2.622-627.1983

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  19 in total

1.  A comparative study of starch hydrolysis by fecal streptococci employing plate and tube techniques.

Authors:  M T Pavlova; W Litsky; F J Francis
Journal:  Health Lab Sci       Date:  1971-04

2.  Concepts of fecal streptococci in stream pollution.

Authors:  E E Geldreich; B A Kenner
Journal:  J Water Pollut Control Fed       Date:  1969-08

Review 3.  Media and methods for isolation and enumeration of the enterococci.

Authors:  P A Hartman; G W Reinbold; D S Saraswat
Journal:  Adv Appl Microbiol       Date:  1966       Impact factor: 5.086

4.  Two improved media for isolating and enumerating enterococci in certain frozen foods.

Authors:  R V Lachica; P A Hartman
Journal:  J Appl Bacteriol       Date:  1968-03

5.  Rapid identification of Enterobacteriaceae with microbial enzyme activity profiles.

Authors:  J H Godsey; M R Matteo; D Shen; G Tolman; J R Gohlke
Journal:  J Clin Microbiol       Date:  1981-03       Impact factor: 5.948

6.  A new approach for quantitative and semi-quantitative determinations of enzymatic activities with simple laboratory equipment. Detection of -amylase.

Authors:  M Ceska
Journal:  Clin Chim Acta       Date:  1971-06       Impact factor: 3.786

7.  Recognition of group D streptococcal species of human origin by biochemical and physiological tests.

Authors:  R R Facklam
Journal:  Appl Microbiol       Date:  1972-06

8.  Association of Streptococcus bovis with carcinoma of the colon.

Authors:  R S Klein; R A Recco; M T Catalano; S C Edberg; J I Casey; N H Steigbigel
Journal:  N Engl J Med       Date:  1977-10-13       Impact factor: 91.245

9.  Gentamicin-based medium for the isolation of group D streptococci and application of the medium to water analysis.

Authors:  L S Donnelly; P A Hartman
Journal:  Appl Environ Microbiol       Date:  1978-03       Impact factor: 4.792

10.  Gentamicin-thallous-carbonate medium for isolation of fecal streptococci from foods.

Authors:  T S Thian; P A Hartman
Journal:  Appl Environ Microbiol       Date:  1981-03       Impact factor: 4.792

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  4 in total

Review 1.  Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors:  M Manafi; W Kneifel; S Bascomb
Journal:  Microbiol Rev       Date:  1991-09

2.  Rapid fluorescence method for screening Salmonella spp. from enteric differential agars.

Authors:  P M Aguirre; J B Cacho; L Folgueira; M López; J García; A C Velasco
Journal:  J Clin Microbiol       Date:  1990-01       Impact factor: 5.948

Review 3.  Use of enzyme tests in characterization and identification of aerobic and facultatively anaerobic gram-positive cocci.

Authors:  S Bascomb; M Manafi
Journal:  Clin Microbiol Rev       Date:  1998-04       Impact factor: 26.132

4.  Comparison of fluorescent gentamicin-thallous-carbonate and KF streptococcal agars to enumerate enterococci and fecal streptococci in meats.

Authors:  L M Knudtson; P A Hartman
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

  4 in total

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