| Literature DB >> 6827103 |
A W Cripps, S H Neoh, I J Smart.
Abstract
A simple method is described for the preparation of highly purified IgA and IgM from small volumes of human serum. Enriched IgM and IgA fractions were prepared by precipitation with 7% (w/v) and 14% (w/v) polyethylene glycol respectively. This was followed by affinity chromatography and gel filtration. The final recovery of both IgA and IgM was approximately 30%. The purified preparations obtained were characterized by immunoelectrophoresis, double immunodiffusion, radial immunodiffusion, radioimmunoassay and gel filtration.Entities:
Mesh：
Substances：
Year: 1983 PMID： 6827103 DOI： 10.1016/0022-1759(83)90078-9
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303