Literature DB >> 6823553

Attogram detection limit for aqueous dye samples by laser-induced fluorescence.

N J Dovichi, J C Martin, J H Jett, R A Keller.   

Abstract

A modified flow cytometer has been used to detect attogram quantities of aqueous rhodamine 6G by laser-induced fluorescence analysis. A detection limit of 28 attograms (35,000 molecules) was obtained, nearly two orders of magnitude better than earlier measurements. The detection limit in concentration units was 1.4 x 10(-13) mole per liter. During the 1-second measurement period, the total volume sampled was 0.42 microliter. On average, only half a rhodamine 6G molecule was present in the 6-picoliter probed volume.

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Year:  1983        PMID： 6823553     DOI： 10.1126/science.6823553

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  10 in total

1.  Reproducible two-dimensional capillary electrophoresis analysis of Barrett's esophagus tissues.

Authors:  James R Kraly; Megan R Jones; David G Gomez; Jane A Dickerson; Melissa M Harwood; Michael Eggertson; Thomas G Paulson; Carissa A Sanchez; Robert Odze; Ziding Feng; Brian J Reid; Norman J Dovichi
Journal:  Anal Chem       Date:  2006-09-01       Impact factor: 6.986

Review 2.  Development of capillary liquid chromatography: A personal perspective.

Authors:  Milos V Novotny
Journal:  J Chromatogr A       Date:  2017-06-24       Impact factor: 4.759

3.  Simplified sheath flow cuvette design for ultrasensitive laser induced fluorescence detection in capillary electrophoresis.

Authors:  Oluwatosin O Dada; Bonnie J Huge; Norman J Dovichi
Journal:  Analyst       Date:  2012-05-18       Impact factor: 4.616

4.  Nine orders of magnitude dynamic range: picomolar to millimolar concentration measurement in capillary electrophoresis with laser induced fluorescence detection employing cascaded avalanche photodiode photon counters.

Authors:  Oluwatosin O Dada; David C Essaka; Ole Hindsgaul; Monica M Palcic; Jillian Prendergast; Ronald L Schnaar; Norman J Dovichi
Journal:  Anal Chem       Date:  2011-03-16       Impact factor: 6.986

5.  Capillary isoelectric focusing with pH 9.7 cathode for the analysis of gastric biopsies.

Authors:  Lauren M Ramsay; Nathan Cermak; Oluwatosin O Dada; Norman J Dovichi
Journal:  Anal Bioanal Chem       Date:  2011-04-02       Impact factor: 4.142

6.  Preparation and electrophoretic separation of Bodipy-Fl-labeled glycosphingolipids.

Authors:  Scott A Sarver; Richard B Keithley; David C Essaka; Hidenori Tanaka; Yayoi Yoshimura; Monica M Palcic; Ole Hindsgaul; Norman J Dovichi
Journal:  J Chromatogr A       Date:  2012-01-18       Impact factor: 4.759

7.  Quantitative evaluation of the feasibility of sampling the ice plumes at Enceladus for biomarkers of extraterrestrial life.

Authors:  James S New; Bahar Kazemi; Vassilia Spathis; Mark C Price; Richard A Mathies; Anna L Butterworth
Journal:  Proc Natl Acad Sci U S A       Date:  2021-09-14       Impact factor: 12.779

8.  Femtomolar concentration detection limit and zeptomole mass detection limit for protein separation by capillary isoelectric focusing and laser-induced fluorescence detection.

Authors:  Lauren M Ramsay; Jane A Dickerson; Oluwatosin Dada; Norman J Dovichi
Journal:  Anal Chem       Date:  2009-03-01       Impact factor: 6.986

9.  Six orders of magnitude dynamic range in capillary electrophoresis with ultrasensitive laser-induced fluorescence detection.

Authors:  Colin D Whitmore; David Essaka; Norman J Dovichi
Journal:  Talanta       Date:  2009-08-05       Impact factor: 6.057

10.  Internal fluorescence labeling with fluorescent deoxynucleotides in two-label peak-height encoded DNA sequencing by capillary electrophoresis.

Authors:  H R Starke; J Y Yan; J Z Zhang; K Mühlegger; K Effgen; N J Dovichi
Journal:  Nucleic Acids Res       Date:  1994-09-25       Impact factor: 16.971
  10 in total

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