Literature DB >> 6822672

Detection of complement fixation by enzyme linked immunosorbant assay (COMPELISA).

P M Hinchliffe, L Robertson.   

Abstract

A method is described by which complement fixation is detected with an enzyme linked immunosorbant assay (ELISA) technique. The method obviates the need for sensitised sheep red blood cells as an indicator of complement fixation and the titration of complement is not critical. The dose response curve has the advantage of being steep and the test result is read photometrically. As test serum and complement do not react together serum anticomplementary effects are eliminated. The ELISA complement fixation test (COMPELISA) was more sensitive than the conventional CFT for detecting brucella antibodies.

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Year:  1983        PMID： 6822672      PMCID： PMC498114          DOI： 10.1136/jcp.36.1.100

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  2 in total

1.  Physical, chemical, and immunological properties of lipopolysaccharide released from Escherichia coli by ethylenediaminetetraacetate.

Authors:  L Leive; V K Shovlin; S E Mergenhagen
Journal:  J Biol Chem       Date:  1968-12-25       Impact factor: 5.157

2.  CELISA (complement-enzyme linked immuno sorbent assay) a new method for the estimation of complement fixing antibodies; its use for Chagas' disease.

Authors:  A Tandon; H Zahner; G Lämmler
Journal:  Tropenmed Parasitol       Date:  1979-06
  2 in total
  3 in total

1.  Enzyme-linked immunosorbent assay with major outer membrane proteins of Brucella melitensis to measure immune response to Brucella species.

Authors:  S B Hunter; W F Bibb; C N Shih; A F Kaufmann; J R Mitchell; R M McKinney
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

2.  Evidence of udder excretion of Campylobacter jejuni as the cause of milk-borne campylobacter outbreak.

Authors:  D N Hutchinson; F J Bolton; P M Hinchliffe; H C Dawkins; S D Horsley; E G Jessop; P A Robertshaw; D E Counter
Journal:  J Hyg (Lond)       Date:  1985-04

Review 3.  Brucella ceti and brucellosis in cetaceans.

Authors:  Caterina Guzmán-Verri; Rocío González-Barrientos; Gabriela Hernández-Mora; Juan-Alberto Morales; Elías Baquero-Calvo; Esteban Chaves-Olarte; Edgardo Moreno
Journal:  Front Cell Infect Microbiol       Date:  2012-02-06       Impact factor: 5.293
  3 in total

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